Annexin A2 (ANXA2) is an important host factor regulating several key processes in many viruses. To evaluate the potential involvement of ANXA2 in the life cycle of classical swine fever virus (CSFV), an RNA interference (RNAi) approach was utilized. Knockdown of ANXA2 did not impair CSFV RNA replication but significantly reduced CSFV production. A comparable reduction of extracellular and intracellular infectivity levels was detected, indicating that ANXA2 might play a role in CSFV assembly rather than in genome replication and virion release. Furthermore, ANXA2 was found to bind CSFV NS5A, an essential replicase component. Amino acids R338, N359, G378 of NS5A were revealed to be pivotal for the ANXA2-NS5A interaction. Substitutions of these amino acids had no effect on viral RNA replication but substantially reduced CSFV production, which might partly be due to these mutations destroying the ANXA2-NS5A interaction. These results suggested that ANXA2 might participate in CSFV production process by binding NS5A.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/vir.0.000048 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Essential role of -encoded mature NS3 in the genome packaging of classical swine fever virus.
J Virol
December 2024
Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Unlabelled: Classical swine fever virus (CSFV) is a member of the genus within the family . The enveloped particles contain a plus-stranded RNA genome encoding a single large polyprotein. The processing of this polyprotein undergoes dynamic changes throughout the infection cycle.
View Article and Find Full Text PDFSynergistic fermentation of Cordyceps militaris and herbal substrates boosts grower pig antioxidant and immune function.
BMC Vet Res
November 2024
Department of Food Science, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan.
Article Synopsis
- Pathogenic infections pose significant health risks to livestock, leading to the exploration of herbal alternatives like Cordyceps militaris (C. militaris) due to concerns about antibiotic resistance and the traditional use of antibiotic growth promoters.
- The study found that the ethanol extract from C. militaris not only has strong antioxidant properties but also effectively inhibits common pig pathogens and reduces inflammation in pig cells, indicating its potential health benefits.
- Pigs supplemented with C. militaris showed improved growth performance and enhanced immune responses, suggesting its viability as a natural growth promoter in livestock.
CRISPR/Cas9-mediated knockout of STAT1 in porcine-derived cell lines to elucidate the role of STAT1 in autophagy following classical swine fever virus infection.
Front Immunol
November 2024
College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning, Guangxi, China.
Signal transducer and activator of transcription 1 (STAT1) plays a critical role in immune response, human STAT1 as a transcriptional suppressor of autophagy genes and autophagic activity. Classical swine fever virus (CSFV)-infected induce autophagy, leading to immune evasion. However, there are limited reports on the function of porcine STAT1 in autophagy during CSFV infection.
View Article and Find Full Text PDFA strategy for detecting CSFV using DNAzyme-HCR cascade amplification.
Anal Methods
November 2024
Xiangya School of Pharmaceutical Sciences in Central South University, Changsha 410013, Hunan, China.
The Hybridization Chain Reaction (HCR) is an isothermal amplification technique widely used for sensing nucleic acids and small molecules. Despite its effectiveness, conventional linear HCR exhibits relatively slow kinetics and insufficient sensitivity. To address this challenge, we have innovatively combined HCR with DNAzyme technology to enhance nucleic acid detection.
View Article and Find Full Text PDFPRRSV infection inhibits CSFV C-strain replication via GSDMD-mediated pyroptosis.
Vet Microbiol
November 2024
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Classical swine fever virus (CSFV) and porcine productive and respiratory syndrome virus (PRRSV) both are significant infectious pathogens in pigs and pose great threats to the healthy development of the pig industry. PRRSV infection often reduces the antibody level of the CSFV attenuated vaccine and even leads to immune failure. In order to elucidate the potential mechanism of CSFV proliferation inhibition by PRRSV and screen out drugs that enhance the vaccine immune effect, we conducted experiments in the PAM39 cell line that can simultaneously support both PRRSV and CSFV infection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!