Background: Cardiovascular disease (CVD) is one of the major causes of death worldwide. Numerous genetic risk factors in lipid metabolism, including mutations of LDLR, APOB, and PCSK9, as well as polymorphisms of CETP and APOE, have been found to associate with CVD.
Methods: In this study, a two-dye based arrayed primer extension (APEX) microarray assay for simultaneous multigene (LDLR, APOB, PCSK9, CETP, and APOE) detection was developed. The DNA templates, originating from 1 DNA sample of known genotype and 7 blind DNA samples, were amplified by uniplex PCR.
Results: Optimized conditions for the APEX reaction were determined to include a hybridization temperature of 55°C and a DNA template size of 50-150bp. The total assay including PCR, purification, fragmentation, APEX reaction, and image analysis could be performed in 6h. In total, 48 genotypes were identified among 8 individual DNA samples by APEX analysis.
Conclusions: The data suggest that this APEX microarray offers a robust, fast, and versatile option for screening these genotypes in hypercholesterolemia patients.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cca.2015.01.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy.
Science
January 2025
Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
Elucidating the interaction between membrane proteins and antibodies requires whole-cell imaging at high spatiotemporal resolution. Lattice light-sheet (LLS) microscopy offers fast volumetric imaging but suffers from limited spatial resolution. DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) achieves molecular resolution but is restricted to two-dimensional imaging owing to long acquisition times.
View Article and Find Full Text PDFSensitive and simple multi-ion detection using organic nanocrystal enrichment in paper analytical devices.
Anal Chim Acta
September 2023
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, 980-8577, Japan; Department of Chemistry, Graduate School of Science, Tohoku University, Sendai, 980-8578, Japan; Department of Chemistry, Tokyo Institute of Technology, Tokyo, Japan. Electronic address:
Microfluidic paper analytical devices (μPADs) are among the most promising platforms for heavy metal ion analysis. On the other hand, achieving simple and highly sensitive analysis of μPADs is challenging. In this study, we developed a simple enrichment method for sensitive multi-ion detection utilizing water-insoluble organic nanocrystals accumulated on μPAD.
View Article and Find Full Text PDFA clinical evaluation of a permanent hair dye designed to reduce allergic contact dermatitis and hair damage.
J Cosmet Dermatol
September 2022
Dermatology Consulting Services, PLLC, High Point, North Carolina, USA.
Background: Permanent hair dye is the most commonly used anti-aging procedure used by both men and women. However, permanent hair dye can cause irritant contact dermatitis due to ammonia and allergic contact dermatitis due to paraphylenediamine (PPD).
Methods: This research examined an ammonia-free and PPD-free permanent hair dye in 50 ethnically diverse females 21-91 years of age who were current users of permanent hair dyes.
Monitoring coastal water flow dynamics using sub-daily high-resolution SkySat satellite and UAV-based imagery.
Water Res
July 2022
Hydrology, Agricultural and Land Observation, Water Desalination and Reuse Center, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
Sub-daily tracking of dynamic features and events using high spatial resolution satellite imagery has only recently become possible, with advanced observational capabilities now available through tasking of satellite constellations. Here, we provide a first of its kind demonstration of using sub-daily 0.50 m resolution SkySat imagery to track coastal water flows, combining these data with object-based detection and a machine-learning approach to map the extent and concentration of two dye plumes.
View Article and Find Full Text PDFAre all charge-transfer parameters created equally? A study of functional dependence and excited-state charge-transfer quantification across two dye families.
Phys Chem Chem Phys
September 2021
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Small molecule organic dyes have many potential uses in medicine, textiles, forensics, and light-harvesting technology. Being able to computationally predict the spectroscopic properties of these dyes could greatly expedite screening efforts, saving time and materials. Time-dependent density functional theory (TD-DFT) has been shown to be a good tool for this in many instances, but characterizing electronic excitations with charge-transfer (CT) character has historically been challenging and can be highly sensitive to the chosen exchange-correlation functional.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!