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A high-throughput screening method for determining the substrate scope of nitrilases. | LitMetric
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A high-throughput screening method for determining the substrate scope of nitrilases.

Gary W Black Nicola L Brown Justin J B Perry P David Randall Graeme Turnbull Meng Zhang

Chem Commun (Camb)

Industrial Biotechnology Research Group, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.

Published: February 2015

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

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 Source
http://dx.doi.org/10.1039/c4cc06021kDOI Listing

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