Decreased deformability of lymphocytes in chronic lymphocytic leukemia.

Sci Rep

1] Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada [2] Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada [3] Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, Canada.

Published: January 2015

AI Article Synopsis

  • This study investigates changes in stiffness and deformability of lymphocytes in patients with chronic lymphocytic leukemia (CLL) at the single-cell level.
  • A microfluidic device was used to measure the volume and transit time of individual lymphocytes from both healthy individuals and CLL patients, revealing that lymphocytes from CLL patients are stiffer (and less deformable) than those from healthy samples.
  • This finding contrasts with other metastatic cells, such as those from breast and lung cancers, which typically show reduced stiffness as metastasis advances.

Article Abstract

This paper reports the first study of stiffness/deformability changes of lymphocytes in chronic lymphocytic leukemia (CLL) patients, demonstrating that at the single cell level, leukemic metastasis progresses are accompanied by biophysical property alterations. A microfluidic device was utilized to electrically measure cell volume and transit time of single lymphocytes from healthy and CLL patients. The results from testing thousands of cells reveal that lymphocytes from CLL patients have higher stiffness (i.e., lower deformability), as compared to lymphocytes in healthy samples, which was also confirmed by AFM indentation tests. This observation is in sharp contrast to the known knowledge on other types of metastatic cells (e.g., breast and lung cancer cells) whose stiffness becomes lower as metastasis progresses.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287721PMC
http://dx.doi.org/10.1038/srep07613DOI Listing

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