We synthesized a cytoprotective magnetic nanoparticle by reacting a maleimide functionalized Feraheme (FH) with a disulfide linked dimer of a polyhis tagged annexin V. Following reductive cleavage of disulfide, the resulting annexin-nanoparticle (diameter = 28.0 ± 2.0 nm by laser light scattering, 7.6 annexin's/nanoparticle) was cytoprotective to cells subjected to plasma membrane disrupting chemotherapeutic or mechanical stresses, and significantly more protective than the starting annexin V. Annexin-nanoparticles provide an approach to the design of nanomaterials which antagonize the plasma membrane permeability characteristic of necrosis and which may have applications as cytoprotective agents.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312233 | PMC |
| http://dx.doi.org/10.1039/c4nr06861k | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Highly selective chemical modification of poly-His tagged peptides and proteins.
Methods Enzymol
June 2024
Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark. Electronic address:
Chemical modifications to proteins have wide applications. They may be used in, for example, the production of biopharmaceuticals and fluorescent probes. Despite their importance, highly regioselective chemical protein modifications are often challenging to achieve.
View Article and Find Full Text PDFEngineering Polyhistidine Tags on Surface Proteins of : Impact of Localization on the Binding and Recovery of Divalent Metal Cations.
ACS Appl Mater Interfaces
March 2022
Department of Chemical Engineering, Columbia University, 500 West 120th Street, New York, New York 10027, United States.
Metal processing using microorganisms has many advantages including the potential for reduced environmental impacts as compared to conventional technologies.is an iron- and sulfur-oxidizing chemolithoautotroph that is known to participate in metal bioleaching, and its metabolic capabilities have been exploited for industrial-scale copper and gold biomining. In addition to bioleaching, microorganisms could also be engineered for selective metal binding, enabling new opportunities for metal bioseparation and recovery.
View Article and Find Full Text PDFNovel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor.
Int J Mol Sci
June 2021
Department of Physiology, Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three forms: as two membrane-spanning proteins hSCF248 and hSCF229 and truncated soluble -terminal protein hSCF164.
View Article and Find Full Text PDFSystematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Protein Expr Purif
December 2018
CUO-Recherche, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada. Electronic address:
Purification of recombinant proteins is often achieved using a purification tag which can be located either at the N- or C-terminus of a passenger protein of interest. Many purification tags exist and their advantages and limitations are well documented. However, designing fusion proteins can be a challenging task to get a fully expressed, soluble and highly purified passenger protein.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!