AI Article Synopsis
- The study aimed to explore how Equol influences gene and protein expression related to testosterone synthesis and Vimentin in mouse testes during early development.
- Testes from perinatal mice were cultured in various concentrations of Equol to assess morphological changes and measure specific protein and gene expressions through techniques like HE staining and real-time PCR.
- Results indicated no significant morphological changes or mRNA expression differences, but protein levels of key enzymes and Vimentin were affected by Equol, particularly showing an increase at 0.10 μmol/L and a decrease at 10.00 μmol/L, suggesting potential negative impacts on testosterone production.
Article Abstract
Objective: To investigate the effects of Equol on genes and protein expression of testosterone synthesis related enzymes and Vimentin in testis of perinatal mice in vitro.
Methods: Testes were isolated and cultured in infiltrating type rotating device for 72 h. The testes were randomly divided into five groups and treated with Equol (DMSO control, 0.01, 0.10, 1.00, 10.00 μmol/L Equol). Morphological changes were observed by HE staining under optical microscope. Expressions of 3β-hydroxysteroid dehydrogenase (3β-HSD),P450 side-chain cleavage enzyme (P450scc), Vimentin were detected by real-time PCR and immunohistochemistry.
Results: No apparent morphological change in testes was observed compared to control group. The mRNA expression of 3β HSD, P450Scc, Vimentin show no statistical significance(P> 0. 05) in all Equol group, while the protein expressions of 3β-HSD, Vimentin, P450scc increased in 0. 10 μmol/L and decreased in 10.00 μmol/L Equol group.
Conclusion: Equol exposure can affect 3β-HSD, P450Scc, Vimentin expression in testes in vitro, means Equol may have potential adverse effects on testosterone production and spermatogenesis.
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View Article and Find Full Text PDF[The effects of equol on enzymes related to testosterone synthesis and vimentin in the testis of perinatal mice by organ culture in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban
November 2014
Department of Nutrition and Food Hygiene, Sichuan University, Chengdu, China.
Article Synopsis
- The study aimed to explore how Equol influences gene and protein expression related to testosterone synthesis and Vimentin in mouse testes during early development.
- Testes from perinatal mice were cultured in various concentrations of Equol to assess morphological changes and measure specific protein and gene expressions through techniques like HE staining and real-time PCR.
- Results indicated no significant morphological changes or mRNA expression differences, but protein levels of key enzymes and Vimentin were affected by Equol, particularly showing an increase at 0.10 μmol/L and a decrease at 10.00 μmol/L, suggesting potential negative impacts on testosterone production.
[The effects of di-(2-ethylhexyl) phthalate (DEHP) in testosterone synthesis and its molecular mechanisms in the fetal testis of male mouse by organ culture in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban
July 2013
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Article Synopsis
- The study aimed to evaluate how DEHP impacts testosterone production and related gene expression in the fetal testis of male mice using organ culture techniques.
- Results indicated increased testosterone levels at lower DEHP concentrations (0.1, 1.0 micromol/L), but decreased levels at higher concentration (100.0 micromol/L) after 48 and 72 hours of exposure.
- Changes were observed in gene expression related to testosterone synthesis, particularly a significant decrease in key genes at 1.0 and 10.0 micromol/L, suggesting that DEHP may disrupt testosterone production through its effects on gene regulation.
[Effects of bisphenol A on testis testosterone synthesis in mouse cultured in vitro].
Wei Sheng Yan Jiu
July 2013
West China School of Public Health Sichuan University, Chengdu 610041, China.
Objective: To investigate the effects of BPA on testosterone synthesis and the related genes expression in the fetal testis of male mouse by organ culture in vitro.
Methods: The testis tissues cultured in vitro were randomly divided into five groups, DMSO control group and 4 different concentration of BPA groups (10(-7), 10(-6), 10(-5) and 10(-4) mol/L respectively). The culture medium and gas were changed every 24 h.
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