This paper considers the potential of CMOS-based nanopore measurement systems for high-speed molecular recording and DNA sequencing in particular. As such it approximates the signal fidelity limitations of a CMOS-based nanopore readout channel and discusses the means by which throughput could be increased through the use of a parallel CMOS readout system.
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http://dx.doi.org/10.1109/EMBC.2014.6943901 | DOI Listing |
J Phys Chem A
May 2024
Imec, Kapeldreef 75, B-3001 Leuven, Belgium.
Nanopore field-effect transistor (NP-FET) devices hold great promise as sensitive single-molecule sensors, which provide CMOS-based on-chip readout and are also highly amenable to parallelization. A plethora of applications will therefore benefit from NP-FET technology, such as large-scale molecular analysis (e.g.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
November 2016
Department of Genetics, Harvard Medical School, Boston, MA 02115;
Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides with polymer tags that allow discrimination of the nucleotides in a biological nanopore. Here, we designed and covalently coupled a DNA polymerase to an α-hemolysin (αHL) heptamer using the SpyCatcher/SpyTag conjugation approach.
View Article and Find Full Text PDFAnnu Int Conf IEEE Eng Med Biol Soc
July 2016
This paper considers the potential of CMOS-based nanopore measurement systems for high-speed molecular recording and DNA sequencing in particular. As such it approximates the signal fidelity limitations of a CMOS-based nanopore readout channel and discusses the means by which throughput could be increased through the use of a parallel CMOS readout system.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!