AI Article Synopsis
- Antiendothelial cell antibodies (AECAs) have been found in both autoimmune and inflammatory diseases, and this study specifically focused on AECAs targeting human brain microvascular endothelial cells (HBMEC).
- The researchers detected 250 antigen spots reacting with AECAs in serum samples from 30 healthy subjects, identifying 10 common spots present in over 25% of samples.
- By using mass spectrometry, they identified 7 key proteins—such as glucose-regulated protein and annexin A2—that are likely the main targets for natural AECAs in these individuals.
Article Abstract
Antiendothelial cell antibodies (AECAs) have been detected in patients who have autoimmune and inflammatory diseases. Previous studies showed that AECAs against human umbilical vein endothelial cells were detected in healthy subjects. In the present study, we evaluated AECAs against human brain microvascular endothelial cells (HBMEC) in serum. We detected 250 antigen spots that reacted with AECAs in serum samples from 30 healthy subjects by 2-dimensional immunoblot using primary cultured HBMEC as the antigen source. There were 10 spots that corresponded to common target antigen spots and reacted with AECAs in serum samples from > 25% of the 30 healthy subjects. We identified 7 proteins that corresponded to 8 of the 10 spots by mass spectrometry: 78-kDa glucose-regulated protein, dihydropyrimidinase-related protein 2, heterogeneous nuclear ribonucleoprotein L, vimentin, perilipin 3, alpha-enolase, and annexin A2. The results suggest that these 7 HBMEC proteins are major target antigens of natural AECAs.
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| http://dx.doi.org/10.2174/1567202612666150107161847 | DOI Listing |
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