Transition-metal-mediated uncaging in living human cells—an emerging alternative to photolabile protecting groups.

Curr Opin Chem Biol

Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35043 Marburg, Germany; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, People's Republic of China. Electronic address:

Published: April 2015

Photolabile protecting groups have been widely used for activation strategies of caged substrates within living cells. However, an alternative uncaging method in which, instead of light, chemical compounds are used as activators (chemical uncaging) is still in its infancy. The recent advances in bioorthogonal reactions mediated by transition metals have shown that bioorthogonal catalysts have the potential to yield such a chemical activator. By now we have seen transition metal compounds that activate caged enzymes, toxigenic prodrugs and other small molecules such as fluorophores within living human cells. In this review we will focus on metal catalysts based on palladium, ruthenium and iron and we will mainly discuss their biocompatibility and catalytic efficiency in uncaging reactions within biological environments.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpa.2014.12.021DOI Listing

Publication Analysis

Top Keywords

living human
8
photolabile protecting
8
protecting groups
8
transition-metal-mediated uncaging
4
uncaging living
4
human cells—an
4
cells—an emerging
4
emerging alternative
4
alternative photolabile
4
groups photolabile
4

Similar Publications

Background: No study has assessed the impact of flavor capsule cigarettes (FCCs) on smoking cessation. Thus, the purpose of this exploratory study was to assess (1) the sociodemographic and smoking-related characteristics associated with using FCCs, and (2) the preliminary impact of FCCs on smoking cessation.

Methods: This study is a secondary data analysis of a single-arm study with 100 individuals living in Mexico who smoked and received a smoking cessation mHealth intervention and pharmacotherapy support.

View Article and Find Full Text PDF

Background: Despite growing research on the daily life of people with advanced cancer, more specific knowledge is needed about the specific strategies these people use to manage everyday activities.

Purpose: This study explores how people with advanced cancer manage their everyday activities and describe their specific strategies.

Methods: The qualitative study was designed with an explorative approach.

View Article and Find Full Text PDF

Background: Recurrent early pregnancy loss [rEPL] is a traumatic experience, marked by feelings such as grief and depression, and often anxiety. Despite this, the psychological consequences of rEPL are often overlooked, particularly when considering future reproductive health or approaching subsequent pregnancies. The SARS-CoV-2 pandemic led to significant reconfiguration of maternity care and a negative impact on the perinatal experience, but the specific impact on women's experience of rEPL has yet to be explored.

View Article and Find Full Text PDF

Background: Most existing studies focus on either the relationship between sarcopenia and depressive symptoms or sarcopenia and cognitive functions. Few studies have examined depressive symptoms and cognitive functions simultaneously. This study aimed to analyze the relationship between sarcopenia and both depressive symptoms and cognitive functions in the elderly by introducing activities of daily living to explore the mediating role of activities of daily living between sarcopenia and mental health.

View Article and Find Full Text PDF

Exploring imitation of within hand prehensile object manipulation using fMRI and graph theory analysis.

Sci Rep

January 2025

Support Centre for Advanced Neuroimaging (SCAN), Institute for Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.

This study aims to establish an imitation task of multi-finger haptics in the context of regular grasping and regrasping processes during activities of daily living. A video guided the 26 healthy, right-handed volunteers through the three phases of the task: (1) fixation of a hand holding a cuboid, (2) observation of the sensori-motor manipulation, (3) imitation of that motor action. fMRI recorded the task; graph analysis of the acquisitions revealed the associated functional cerebral connectivity patterns.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!