AI Article Synopsis

  • - Trypanosoma cruzi causes Chagas disease, affecting around 8 million people globally, leading to significant inflammation that helps control the disease.
  • - The study tested a drug called NO-indomethacin on mice infected with T. cruzi, to see its impact on parasite levels, cell invasion, and oxidative stress.
  • - Results showed that NO-indomethacin treatment increased parasitemia, mortality, and oxidative stress while inhibiting nitric oxide production in macrophages, indicating caution is needed in developing NO-NSAID therapies for infections.

Article Abstract

Trypanosoma cruzi is the causative agent of Chagas disease. Approximately 8 million people are thought to be affected with this disease worldwide. T. cruzi infection causes an intense inflammatory response, which is critical for the control of parasite proliferation and disease development. Nitric oxide-donating nonsteroidal anti-inflammatory drugs (NO-NSAIDs) are an emergent class of pharmaceutical derivatives with promising utility as chemopreventive agents. In this study, we investigated the effect of NO-indomethacin on parasite burden, cell invasion, and oxidative stress in erythrocytes during the acute phase of infection. NO-indomethacin was dissolved in dimethyl formamide followed by i.p. administration of 50 ppm into mice 30 min after infection with 5×10(3) blood trypomastigote forms (Y strain). The drug was administered every day until the animals died. Control animals received 100 μL of drug vehicle via the same route. Within the NO-indomethacin-treatment group, parasitemia and mortality (100%) were higher and oxidative stress in erythrocytes, anemia, and entry of parasites into macrophages were significantly greater than that seen in controls. Increase in the entry and survival of intracellular T. cruzi was associated with inhibition of nitric oxide production by macrophages treated with NO-indomethacin (2.5 μM). The results of this study provide strong evidence that NO-NSAIDs potently inhibit nitric oxide production, suggesting that NO-NSAID-based therapies against infections would be difficult to design and would require caution.

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http://dx.doi.org/10.1016/j.cbi.2014.12.024DOI Listing

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