AI Article Synopsis
- Wnt/β-catenin signaling is crucial for maintaining tissue health and repair in animals, particularly in planarians, which can regenerate effectively.
- Researchers identified numerous transcripts influenced by β-catenin through RNA sequencing, noting differential expression in muscle and stem cells.
- One specific gene, teashirt (tsh), was found to play a key role in regeneration, as its depletion led to abnormal head formation in planarians, indicating a strong connection between β-catenin activity and the regulation of cell identity during tissue regeneration.
Article Abstract
Wnt/β-catenin signaling regulates tissue homeostasis and regeneration in metazoans. In planarians-flatworms with high regenerative potential-Wnt ligands are thought to control tissue polarity by shaping a β-catenin activity gradient along the anterior-posterior axis, yet the downstream mechanisms are poorly understood. We performed an RNA sequencing (RNA-seq)-based screen and identified hundreds of β-catenin-dependent transcripts, of which several were expressed in muscle tissue and stem cells in a graded fashion. In particular, a teashirt (tsh) ortholog was induced in a β-catenin-dependent manner during regeneration in planarians and zebrafish, and RNAi resulted in two-headed planarians. Strikingly, intact planarians depleted of tsh induced anterior markers and slowly transformed their tail into a head, reminiscent of β-catenin RNAi phenotypes. Given that β-catenin RNAi enhanced the formation of muscle cells expressing anterior determinants in tail regions, our study suggests that this pathway controls tissue polarity through regulating the identity of differentiating cells during homeostasis and regeneration.
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| http://dx.doi.org/10.1016/j.celrep.2014.12.018 | DOI Listing |
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