Cancer heterogeneity has received considerable attention for its role in tumor initiation and progression, and its implication for diagnostics and therapeutics in the clinic. To facilitate a cellular heterogeneity study in a low cost and highly efficient manner, we present a microfluidic platform that allows traceable clonal culture and characterization. The platform captures single cells into a microwell array and cultures them for clonal expansion, subsequently allowing on-chip characterization of clonal phenotype and response against drug treatments. Using a heterogeneous prostate cancer model, the PC3 cell line, we verified our prototype, identifying three different sub-phenotypes and correlating their clonal drug responsiveness to cell phenotype.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232586 | PMC |
| http://dx.doi.org/10.1063/1.4900823 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Establishment of a novel clonal GFP-expressing transgenic ginbuna crucian carp.
Dev Comp Immunol
November 2024
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan.
The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii, a naturally occurring gynogenetic fish, is suitable for cell transplantation studies to reveal the roles of stem cells and immune cells. To ensure long-term traceability of donor cells within recipient fish, we have established a transgenic ginbuna line that expresses green fluorescent protein (GFP). The Xenopus laevis ef1a promoter was introduced for regulating GFP expression.
View Article and Find Full Text PDFPrevalence and Genomic Characterization of Multidrug-Resistant Serovar Kentucky Sequence Type 198 Circulating - Beijing Municipality, China, 2016-2023.
China CDC Wkly
August 2024
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing, China.
Article Synopsis
- - The study focuses on a highly resistant strain of Salmonella Kentucky (ST198) that poses a global health threat due to its multidrug resistance, particularly against fluoroquinolones.
- - Researchers analyzed 54 Kentucky isolates from clinical and food sources in Beijing between 2016 and 2023 using genome sequencing and antibiotic testing, revealing various resistance patterns and mutations.
- - Findings show significant genetic diversity within the Kentucky population, highlighting the need for enhanced monitoring and control strategies to combat antimicrobial resistance (AMR) in both food and human health contexts.
Development of Traceable Mouse Models of Advanced and Metastatic Bladder Cancer.
Cancers (Basel)
June 2024
Department of Oncology, Lausanne University Hospital (CHUV), University of Lausanne, 1015 Lausanne, Switzerland.
Bladder cancer (BC) is the fourth most common cancer in men, with a poor patient prognosis for advanced disease. The poor survival of patients with muscle-invasive bladder cancer (MIBC) and metastatic status emphasizes the urgent need to develop new therapies. Lacking in the field of BC is the availability of relevant advanced BC mouse models, especially metastatic ones, that accurately recapitulate the complexities of human pathology to test and study new therapeutic strategies.
View Article and Find Full Text PDFEmergence of ST1193 Clone in Maternal and Neonatal ESBL-Producing Isolates.
Infect Drug Resist
October 2023
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China.
Objective: The emerging epidemic of extended-spectrum β-lactamase-producing (ESBL-EC) is a global public health crisis. ESBL-EC infections are increasing worldwide and contribute to morbidity and mortality among newborn infants. However, the antimicrobial resistance characteristics and clonal transmission of maternal and neonatal ESBL-EC isolates need to be further deciphered.
View Article and Find Full Text PDFGenomic Analysis and Antimicrobial Resistance of Isolated from Diarrheal Patients - Beijing Municipality, China, 2019-2021.
China CDC Wkly
May 2023
Beijing Center for Disease Prevention and Control, Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing, China.
Article Synopsis
- The study focuses on the genetic diversity, virulence factors, and antimicrobial resistance of a leading cause of bacterial gastroenteritis during a 3-year surveillance in Beijing, China.
- Researchers analyzed 184 clinical isolates, identifying 71 genetic types and observing high resistance rates to specific antibiotics, particularly quinolones and tetracycline.
- Findings highlight not only significant genetic diversity and multidrug resistance among the isolates but also a concerning trend in rising antibiotic resistance as well as various virulence traits related to disease severity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!