Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.
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http://dx.doi.org/10.1534/g3.114.015701 | DOI Listing |
Microbiol Resour Announc
December 2024
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
is a non-Saccharomyces yeast that is widely used in winemaking due to its ability to ferment malic acid, thus improving organoleptic properties of wine. We report the draft genome sequence of strain I-540, isolated from grape must in Russia.
View Article and Find Full Text PDFJ Biochem
December 2024
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, Japan.
Nuclear pore complexes (NPCs) act as gateways across the nuclear envelope for molecular transport between the nucleus and the cytoplasm in eukaryotes. NPCs consist of several subcomplexes formed by multiple copies of approximately 30 different proteins known as nucleoporins (Nups). In the fission yeast Schizosaccharomyces pombe, the NPC structure is unique, particularly in its outer ring subcomplexes, where the cytoplasmic and nucleoplasmic outer rings are composed of distinct sets of proteins.
View Article and Find Full Text PDFJ Photochem Photobiol B
December 2024
Microbiology Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia. Electronic address:
This study evaluated the photoprotective and antioxidant properties of eumelanin derived from Streptomyces lasalocidi NTB 42 (eumelanin NTB 42). This study also investigated the cellular-level photoprotective effects of eumelanin using Schizosaccharomyces pombe ARC039 as a model organism and its ability to enhance the Sun Protection Factor (SPF) of commercial sunscreens. The thermal and light stability and total phenolic and flavonoid contents were analyzed.
View Article and Find Full Text PDFFood Chem
December 2024
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus of Gualtar, University of Minho, 4710-057 Braga, Portugal; IBS, Institute of Science and Innovation for Bio-Sustainability, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address:
Microalgae are emerging as valuable sources of bioactive compounds. This study evaluates hexane extracts from Thalassiosira sp. and Raphidonema sp.
View Article and Find Full Text PDFFood Chem X
December 2024
Department of Chemistry and Food Technology, Polytechnic University of Madrid, Ciudad Universitaria, S/N, 28040 Madrid, Spain.
Most commercially available red wines undergo alcoholic fermentation by yeasts, followed by a second fermentation with the lactic acid bacteria once the initial process is complete. However, this traditional approach can encounter complications in specific scenarios. These situations pose risks such as stalled alcoholic fermentation or the growth of undesirable bacteria while the process remains incomplete, leaving residual sugars in the wine.
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