AI Article Synopsis
- MMP-2 is a marker in metastatic cancer tissues linked to poor prognosis and serves as a target for tumor imaging.
- Scientists created a protein nanocage that has a peptide (CTT) which binds to MMP-2.
- These engineered nanocages can specifically accumulate in tumors, allowing for imaging of cancer activity using near-infrared fluorescence, potentially aiding in noninvasive detection methods.
Article Abstract
Matrix metalloproteinase 2 (MMP-2) in metastatic cancer tissue, which is associated with a poor prognosis, is a potential target for tumor imaging in vivo. Here, we describe a metastatic cancer cell-targeted protein nanocage. An MMP-2-binding peptide, termed CTT peptide (CTTHWGFTLC), was conjugated to the surface of a naturally occurring heat shock protein nanocage by genetic modification. The engineered protein nanocages showed a binding affinity for MMP-2 and selective uptake in cancer cells that highly expressed MMP-2 in vitro. In near-infrared fluorescence imaging, the nanocages showed specific and significant accumulation in tumor tissue after intravenous injection in vivo. These protein nanocages conjugated with CTT peptide could be potentially applied to a noninvasive near-infrared fluorescence detection method for imaging gelatinase activity in metastatic tumors in vivo.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307240 | PMC |
| http://dx.doi.org/10.3390/ijms16010148 | DOI Listing |
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