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Population identification of Sarcoptes hominis and Sarcoptes canis in China using DNA sequences. | LitMetric

AI Article Synopsis

  • - The study investigates the species classification of Sarcoptes mites that infect humans (S. hominis) and dogs (S. canis) in China by analyzing genetic material from both types of mites.
  • - Analysis of mitochondrial DNA (mtDNA) revealed five distinct groups within the Sarcoptes family, identifying four variants of S. hominis and one for S. canis, while showing that S. canis mites from different regions share closer genetic relationships.
  • - The findings indicate that the 317-bp mtDNA cox1 sequence serves as a reliable DNA barcode for accurately identifying Sarcoptes mites, highlighting geographical differences among variants of S. hominis but not among S. canis.

Article Abstract

There has been no consistent conclusion on whether Sarcoptes mites parasitizing in humans and animals are the same species. To identify Sarcoptes (S.) hominis and S. canis in China, gDNA was extracted from individual mites (five from patients with scabies and five from dogs with mange) for amplification of rDNA ITS2, mtDNA 16S, and cox1 fragment sequences. Then, the sequences obtained were aligned with those from different hosts and geographical locations retrieved from GenBank and sequence analyses were conducted. Phylogenetic trees based on 317-bp mtDNA cox1 showed five distinctive branches (species) of Sarcoptes mites, four for S. hominis (S. hominis Chinese, S. nr. hominis Chinese, S. hominis Australian, and S. hominis Panamanian) and one for S. animal (S. animal). S. animal included mites from nine animal species, with S. canis China, S. canis Australia, and S. canis USA clustering as a subbranch. Further sequence divergence analysis revealed no overlap between intraspecific (≤ 2.6 %) and interspecific (2.6-10.5 %) divergences in 317-bp mtDNA cox1. However, overlap was detected between intra- and interspecific divergences in 311-bp rDNA ITS2 or 275-bp mtDNA 16S when the divergences exceeded 1.0 %, which resulted in failure in identification of Sarcoptes. The results showed that the 317-bp mtDNA cox1 could be used as a DNA barcode for molecular identification of Sarcoptes mites. In addition, geographical isolation was observed between S. hominis Chinese, S. hominis Australian, and S. hominis Panamanian, but not between all S. canis. S. canis and the other S. animal belonged to the same species.

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http://dx.doi.org/10.1007/s00436-014-4266-1DOI Listing

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