Purpose: To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism.
Methods: Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package.
Results: In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G2+M phase was remarkably lower than that of untransfected group. Cell proliferation index (PI=S+G2M) in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase (ALP) secretion activity and percentage of apoptotic cell notably increased.
Conclusions: ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
ADAM28 dramatically regulates the biological features of human gingival fibroblasts.
Odontology
July 2019
Qingdao Stomatological Hospital, No.17 De-xian Road, Qingdao, 266000, Shandong, People's Republic of China.
This study was to explore the effects of a disintegrin and metalloproteinase 28 (ADAM28) on the proliferation, differentiation, and apoptosis of human gingival fibroblasts (HGFs) and probable mechanism. After ADAM28 antisense oligodeoxynucleotide (AS-ODN) and sense oligodeoxynucleotide (S-ODN) were transfected into HGFs by Lipofectamine 2000, respectively, the expression discrepancies of ADAM28 among various groups were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting. Methabenzthiazuron (MTT) and cell-cycle assays were used to test the HGFs proliferation activity.
View Article and Find Full Text PDF[The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts].
Shanghai Kou Qiang Yi Xue
October 2014
Department of Stomatology, the 401 Hospital of Chinese PLA. Qingdao 266071, Shandong Province,
Purpose: To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism.
Methods: Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
J Endod
March 2011
Institute of Stomatology, General Hospital of Chinese People's Liberation Army, Beijing, China.
Introduction: The purpose of this study was to investigate the influence of a disintegrin and metalloproteinase 28 (ADAM28) on the proliferation, differentiation, and apoptosis of human dental pulp stem cells (HDPSCs) and possible mechanism.
Methods: After ADAM28 eukaryotic plasmid and antisense oligodeoxynucleotides (AS-ODNs) were constructed and respectively transfected into HDPSCs by Lipofectamine 2000, the ADAM28 expression levels among diverse groups were estimated by reverse transcription polymerase chain reaction (RT-PCR) and western blotting. Methabenzthiazuron (MTT) and cell cycle assays were used to test the HDPSCs proliferation activity.
The regulatory role of a disintegrin and metalloproteinase 28 on the biologic property of human periodontal ligament stem cells.
J Periodontol
June 2010
Institute of Stomatology, General Hospital of Chinese People's Liberation Army, Beijing, China.
Background: A disintegrin and metalloproteinase 28 (ADAM28) is considered to be the possible virulence gene for congenital hypoplasia of tooth root. Periodontal ligament stem cells (PDLSCs) are regarded as playing crucial roles in the developing process of the periodontium and are generally used in dental regenerative medicine. This study evaluates the influence of ADAM28 on the biologic property of human PDLSCs (HPDLSCs) and to postulate the possible mechanism of this influence.
View Article and Find Full Text PDF[Effects of ADAM28 AS-ODN on proliferation, differentiation and apoptosis of HPDLSC].
Shanghai Kou Qiang Yi Xue
October 2009
Institute of Stomatology, General Hospital of Chinese PLA, Beijing, China.
Purpose: To investigate the effects of a disintegrin and metalloproteinase 28(ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human periodontal ligament stem cell(HPDLSC) and possible mechanism.
Methods: HPDLSC was isolated, cultured in vitro and identified. Synthetic ADAM28 AS-ODN and S-ODN were transfected into HPDLSC, respectively.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!