J Chromatogr A
School of Mechanical Engineering, Yonsei University, Seoul, Republic of Korea. Electronic address:
Published: January 2015
The isolation and characterization of circulating tumor cells (CTC) is of great importance in cancer diagnosis and prognosis. Highly sensitive detection of CTCs can be very difficult because they are extremely rare (i.e., 1-5 CTCs per 10(9) erythrocytes) in blood. Recently, various devices have been developed that exploit biochemical (affinity-based) and physical (size or density) methods. Antibody-based isolation has its own limitations, as the expression level of the epitopes for an antibody varies due to the heterogeneity of cancer cells. Harsh conditions associated with physical methods can cause the deformation and damage of CTCs during the isolation process. Here, we propose a microfluidic lateral flow filtration (μ-LaFF) chip in which lateral flow was combined with vertical flow into the filter to capture the CTCs gently. The CTCs experienced weak shear flow owing to the lateral flow and traveled alongside the filter channel until finally being captured. The vertical flow in the filter held the captured cells tightly and served as an exit for uncaptured hematological cells (white and red blood cells). From our μ-LaFF chip we obtained a high capture efficiency (95%) and purity (99%), minimizing any damage to the CTCs. Our μ-LaFF technology is expected to be useful in the diagnosis and prognosis of various cancers.
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http://dx.doi.org/10.1016/j.chroma.2014.12.037 | DOI Listing |
Front Microbiol
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Department of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, China.
The soybean mosaic disease-caused by the (SMV)-significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7.
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January 2025
State Key Laboratory of Offshore Oil Exploitation, Beijing 100028, China.
Shale barriers negatively impact thermal recovery processes of oil sand or ultraheavy oil, particularly during the rising stage of SAGD, by affecting oil flow, steam chamber evolution, and heat distribution. Existing mathematical models for the rising stage of SAGD often overlook the influence of shale barriers on the evolution of the steam chamber and heat distribution. This study includes experiments to investigate the impact of a single shale barrier above the production well during the rising stage of the SAGD.
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January 2025
School of Food Science and Engineering, Hainan University, Haikou 570228, China; Laboratory of Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture and Rural Affairs, Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Food Safety Research Institute, HuBei University, Wuhan 430062, China. Electronic address:
Dibutyl phthalate (DBP), a priority pollutant among phthalic acid esters (PAEs) exhibits significant reproductive and respiratory toxicity. In this study, a multifunctional metal-organic frameworks-mediated colorimetric/photothermal immunosensor was established for the quantitative detection of DBP. Firstly, a highly sensitive and specific monoclonal antibody (mAb), designated 3A5, was prepared with a sensitivity IC value of 16.
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January 2025
College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road Yangling, 712100, Shaanxi, China; College of Food Engineering, Ludong University, Yantai, 264025, Shandong, China. Electronic address:
Within the realm of lateral flow assay (LFIA), the conjugation efficiency between signal tracers and antibody constitutes a pivotal determinant for the sensitivity of the detection system. In this study, three-dimensional (3D) complex flower-like MoS self-assembled from 2D MoS, and natural plant polyphenols "Tannic acid" were introduced for surface modification. This composite material exhibits distinct colorimetric signals, excellent monoclonal antibody coupling efficiency, and commendable photothermal properties.
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January 2025
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China. Electronic address:
Cashews are popular snacks but pose risks to allergic individuals due to Ana o 3 protein. Rapid, accurate detection methods for Ana o 3 are limited. This study developed two detection methods for Ana o 3 allergen.
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