AI Article Synopsis
- The study aimed to see if LIF could boost the growth of neural precursor cells (NPCs) and its link to FluoroGold (FG) labeled neurons in mice following spinal cord injury (SCI).
- Methods involved assessing motor skills through tests and labeling neurons and NPCs to estimate their numbers and analyze their correlation.
- Results showed that mice treated with LIF had significantly better locomotor recovery and higher counts of FG-labeled neurons and NPCs compared to the control group, suggesting that LIF could be a potential treatment for enhancing recovery after SCI.
Article Abstract
Objectives: To explore whether LIF could promote the proliferation of neural precursor cells (NPCs) and to analyze the correlation between increased NPCs and FluoroGold (FG) labeled neurons in mice after spinal cord injury (SCI).
Methods: Motor behavior was assessed using Rotarod and Platform Hang tests; neurons in the corticospinal and rubrospinal systems were labeled with FG, NPCs were immustained with nestin-FITC conjugate. The numbers of FG-labeled neurons and NPCs were estimated, and the correlation between FG-labeled neurons and NPCs was assessed.
Results: Mice in the SCI group showed negligible recovery of locomotor behavior; in contrast, mice in the LIF group showed a statically significant improvement. Both FG-labeled neurons and NPCs were significantly increased in the LIF group compared to the SCI group, and this increase in FG-labeled neurons and NPCs showed a clear association above the lesion level.
Conclusions: LIF could promote locomotive behaviors in mice post-SCI by encouraging the proliferation of NPCs; LIF may in fact be a potential cytokine for the induction of NPCs post-SCI.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277544 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116031 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
The nociceptive inputs of the paraventricular hypothalamic nucleus in formalin stimulated mice.
Neurosci Lett
October 2024
Department of Human Anatomy, Histology and Embryology & K. K. Leung Brain Research Centre, Preclinical School of Medicine, The Fourth Military Medical University, Xi'an 710032, China. Electronic address:
The paraventricular hypothalamic nucleus (PVH) is an important neuroendocrine center involved in pain regulation, but the nociceptive afferent routes for the nucleus are still unclear. We examined the profile of PVH receiving injurious information by a combination of retrograde tracing with Fluoro-Gold (FG) and FOS expression induced by formalin stimuli. The result showed that formalin injection induced significantly increased expression of FOS in the PVH, among which oxytocin containing neurons are one neuronal phenotype.
View Article and Find Full Text PDF[Effect of nerve growth factor on elderly degenerative knee osteoarthritis pain].
Zhongguo Gu Shang
January 2024
Department of Traumatology, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.
Article Synopsis
- The study aimed to investigate the impact of nerve growth factor (NGF) antibody on knee osteoarthritis (KOA) pain using an in vitro rat model.
- Thirty male rats were used, with 20 rats induced with OA pain and divided into a control (saline) and a treatment (anti-NGF) group, followed by gait analysis and examination of dorsal root ganglia (DRG) neurons.
- Results showed that the treatment group exhibited improved gait and reduced levels of calcitonin gene-related peptide (CGRP) compared to the control group, suggesting that anti-NGF therapy may be beneficial for managing knee pain in osteoarthritis.
Analgesic effects and arthritic changes following intra-articular injection of diclofenac etalhyaluronate in a rat knee osteoarthritis model.
BMC Musculoskelet Disord
November 2022
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.
Article Synopsis
- - The study tested the effects of a new pain-relieving drug, Diclofenac etalhyaluronate (DF-HA), on osteoarthritis in a rat model using monoiodoacetate injections to induce the condition.
- - Results showed that DF-HA significantly reduced pain sensitivity and inflammation markers in nerve tissues, but did not change the physical deformity of the joints as assessed through imaging and histology.
- - The conclusion highlights that while DF-HA is effective for alleviating osteoarthritic pain, it does not improve the structural damage in the joints.
Dual orexin receptor blocker suvorexant attenuates hypercapnic ventilatory augmentation in mice.
Brain Res
November 2022
Clinical Research Center, Murayama Medical Center, Musashimurayama, Japan.
Suvorexant (Belsomra(R)), a dual orexin receptor antagonist widely used in the treatment of insomnia, inhibits the arousal system in the brain. However, the drug's ventilatory effects have not been fully explored. This study aims to investigate the expression of orexin receptors in respiratory neurons and the effects of suvorexant on ventilation.
View Article and Find Full Text PDFWhole-brain mapping of afferent projections to the suprachiasmatic nucleus of the tree shrew.
Tissue Cell
December 2021
Chinese Academy of Science Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
The suprachiasmatic nucleus (SCN) is essential for the neural control of mammalian circadian timing system. The circadian activity of the SCN is modulated by its afferent projections. In the present study, we examine neuroanatomical characteristics and afferent projections of the SCN in the tree shrew (Tupaia belangeri chinensis) using immunocytochemistry and retrograde tracer Fluoro-Gold (FG).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!