AI Article Synopsis

  • The study investigates mitochondrial genome variations, specifically in the mt-ATP6 and mt-CytB genes, to understand their connection to childhood obesity in Turkish children aged 6-14.
  • Researchers sequenced these genes in 100 obese children and compared the findings with 100 healthy controls, identifying a total of 118 variations.
  • Two specific synonymous substitutions in the mt-ATP6 gene were significantly more common in the obese group, suggesting a link to childhood obesity, while the findings also highlight the need for further research involving larger samples.

Article Abstract

Objective: Due to the importance of energy metabolism in mitochondria, mitochondrial genome variations are evaluated in energy-related diseases such as obesity. To date, several nuclear genes were found to be related to obesity. Our aim in this study was to investigate the presence of polymorphisms in mitochondrial ATPase subunit 6 (mt-ATP6) and cytochrome b (mt-CytB) genes that may be associated with childhood obesity.

Methods: The mt-ATP6 and mt-CytB genes were amplified and entirely sequenced in a series of 100 obese and in an equal number of healthy Turkish children aged between 6-14 years.

Results: A total of 118 synonymous and nonsynonymous variations were detected in the obese and control groups. Only two previously reported synonymous substitutions (mt.8614T>C and mt.8994G>A) in the mt-ATP6 gene were found to be significantly higher in the obese group compared to the control group (p<0.05). In the mt-ATP6 gene, one novel nonsynonymous substitution (mt.8726C>T) and one novel synonymous substitution (mt.9108A>T) were found. In the mt-CytB gene, one nonsynonymous substitution (mt.14880T>C) and two synonymous substitutions (mt.14891C>T and mt.15091C>T) were novel substitutions.

Conclusion: Two synonymous substitutions (mt.8614T>C and mt.8994G>A) in the mt-ATP6 gene may be associated with childhood obesity. Our study provides the first data about mitochondrial genome variations in a Turkish obese population and also the first in obese children. More cases should be screened in obese groups in order to understand the effects of mitochondrial polymorphisms in the development of obesity.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293655PMC
http://dx.doi.org/10.4274/Jcrpe.1601DOI Listing

Publication Analysis

Top Keywords

synonymous substitutions
12
mitochondrial atpase
8
atpase subunit
8
turkish children
8
mitochondrial genome
8
genome variations
8
mt-cytb genes
8
associated childhood
8
substitutions mt8614t>c
8
mt8614t>c mt8994g>a
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!