Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system.

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.