The first data on nucleotide sequences of cytochrome C oxidase I (CO1) gene of Arctic and striped flounders of the Sea of Okhotsk are analyzed. Nucleotide diversity of CO1 gene for 10 individuals of arctic flounder (π = 0,00370) is slightly less than that for 28 individuals of a striped flounder (π = 0,00446), caught in the Taui Lip, the Sea of Okhotsk. The size of the disparity index found on variability of nucleotides at individuals of a polar flounder indicates not selective nature of replacements, whereas at individuals of a striped flounder about a third of replacements is selective. The size of nucleotide distinctions between individuals arctic and striped flounders confirms the specific status of these fishes.

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