AI Article Synopsis
- The study investigated the role of Npas4 in controlling seizures in rats with pilocarpine-induced epilepsy through various protein detection methods.
- Npas4 levels were found to increase during the acute phase of seizures but decreased in the chronic phase, and it was predominantly located in neuronal nuclei and cytoplasm.
- Knocking down Npas4 led to increased seizure frequency and severity, suggesting that Npas4 plays a protective role against seizures in this model.
Article Abstract
To explore the effects of neuronal Per-Arnt-Sim domain protein 4 (Npas4) on seizures in pilocarpine-induced epileptic rats, Npas4 expression was detected by double-label immunofluorescence, immunohistochemistry, and Western blotting in the brains of pilocarpine-induced epileptic model rats at 6 h, 24 h, 72 h, 7 d, 14 d, 30 d, and 60 d after status epilepticus. Npas4 was localized primarily in the nucleus and in the cytoplasm of neurons. The Npas4 protein levels increased in the acute phase of seizures (between 6 h and 72 h) and decreased in the chronic phases (between 7 d and 60 d) in the rat model. Npas4 expression was knocked down by specific siRNA interference. Then, the animals were treated with pilocarpine, and the effects on seizures were evaluated on the 7th day. The onset latencies of pilocarpine-induced seizures were decreased, while the seizure frequency, duration and attack rate increased in these rats. Our study indicates that Npas4 inhibits seizure attacks in pilocarpine-induced epileptic rats.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275263 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115801 | PLOS |
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