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Liquid chromatography with diode array detection combined with spectral deconvolution for the analysis of some diterpene esters in Arabica coffee brew. | LitMetric

AI Article Synopsis

  • The study focused on separating kahweol and cafestol esters from Arabica coffee using liquid chromatography with a diode array detector.
  • By optimizing the detection wavelength to 290 nm, researchers achieved selective quantification of kahweol esters, while spectral deconvolution was employed for cafestol esters.
  • The results showed that both methods for quantifying kahweol esters were effective, with the deconvoluted approach providing slightly better detection limits and reliable repeatability of measurements.

Article Abstract

In this manuscript, the separation of kahweol and cafestol esters from Arabica coffee brews was investigated using liquid chromatography with a diode array detector. When detected in conjunction, cafestol, and kahweol esters were eluted together, but, after optimization, the kahweol esters could be selectively detected by setting the wavelength at 290 nm to allow their quantification. Such an approach was not possible for the cafestol esters, and spectral deconvolution was used to obtain deconvoluted chromatograms. In each of those chromatograms, the four esters were baseline separated allowing for the quantification of the eight targeted compounds. Because kahweol esters could be quantified either using the chromatogram obtained by setting the wavelength at 290 nm or using the deconvoluted chromatogram, those compounds were used to compare the analytical performances. Slightly better limits of detection were obtained using the deconvoluted chromatogram. Identical concentrations were found in a real sample with both approaches. The peak areas in the deconvoluted chromatograms were repeatable (intraday repeatability of 0.8%, interday repeatability of 1.0%). This work demonstrates the accuracy of spectral deconvolution when using liquid chromatography to mathematically separate coeluting compounds using the full spectra recorded by a diode array detector.

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Source
http://dx.doi.org/10.1002/jssc.201401095DOI Listing

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