A rapid antimicrobial susceptibility test based on single-cell morphological analysis.

Sci Transl Med

Center for Nanoparticle Research, Institute for Basic Science, Seoul 151-742, Republic of Korea. Department of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Republic of Korea. Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea. QuantaMatrix Inc., Seoul 151-742, Republic of Korea.

Published: December 2014

AI Article Synopsis

  • A new rapid antibiotic susceptibility test (AST) called single-cell morphological analysis (SCMA) addresses the urgent need for fast antibiotic administration in clinical settings by analyzing single bacterial cell changes under various antimicrobial conditions.
  • The SCMA was validated with multiple bacterial strains and clinical samples, including antibiotic-resistant strains, and compared to the traditional broth microdilution test.
  • Results were achieved in under 4 hours, showing 91.5% agreement with the gold standard, making SCMA a promising tool for timely and accurate antimicrobial susceptibility testing.

Article Abstract

A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. The SCMA was tested with four Clinical and Laboratory Standards Institute standard bacterial strains and 189 clinical samples, including extended-spectrum β-lactamase-positive Escherichia coli and Klebsiella pneumoniae, imipenem-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococci from hospitals. The results were compared with the gold standard broth microdilution test. The SCMA results were obtained in less than 4 hours, with 91.5% categorical agreement and 6.51% minor, 2.56% major, and 1.49% very major discrepancies. Thus, SCMA provides rapid and accurate antimicrobial susceptibility data that satisfy the recommended performance of the U.S. Food and Drug Administration.

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Source
http://dx.doi.org/10.1126/scitranslmed.3009650DOI Listing

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