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Chip-scale, optical microcavity-based biosensors typically employ an ultra-high-quality microcavity and require a precision wavelength-tunable laser for exciting the cavity resonance. For point-of-care applications, however, such a system based on measurements in the spectral domain is prone to equipment noise and not portable. An alternative microcavity-based biosensor that enables a high sensitivity in an equipment-noise-tolerant and potentially portable system is desirable. Here, we demonstrate the proof-of-concept of such a biosensor using a coupled-resonator optical-waveguide (CROW) on a silicon-on-insulator chip. The sensing scheme is based on measurements in the spatial domain, and only requires exciting the CROW at a fixed wavelength and imaging the out-of-plane elastic light-scattering intensity patterns of the CROW. Based on correlating the light-scattering intensity pattern at a probe wavelength with the light-scattering intensity patterns at the CROW eigenstates, we devise a pattern-recognition algorithm that enables the extraction of a refractive index change, Δn, applied upon the CROW upper-cladding from a calibrated set of correlation coefficients. Our experiments using an 8-microring CROW covered by NaCl solutions of different concentrations reveal a Δn of ~1.5 × 10(-4) refractive index unit (RIU) and a sensitivity of ~752 RIU(-1), with a noise-equivalent detection limit of ~6 × 10(-6) RIU.
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http://dx.doi.org/10.1038/srep07528 | DOI Listing |
ACS Photonics
December 2024
Department of Applied Physics, University of Barcelona, 08028 Barcelona, Spain.
The delivery of light over an extended area within a sample forms the basis of biomedical applications that are as relevant as photoacoustic tomography, fluorescence imaging, and phototherapy techniques. However, light scattering limits the ability of these methods to reach deep regions within biological tissues. As a result, their operational range remains confined to superficial areas of samples, posing a significant barrier to effective optical treatment and diagnosis.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
School of Textile Science and Engineering, Xi'an Polytechnic University, Xi'an 710048, Shaanxi, China; Key Laboratory of Functional Textile Material and Product, Xi'an Polytechnic University, Ministry of Education, Xi'an 710048, Shaanxi, China. Electronic address:
We provided an efficient method for preparing fluorescent materials with high specificity. Firstly, the cellulose-based aggregations with adjustable morphologies and sizes were obtained by cross-linking copolymerization and self-assembly. Then, after encapsulating the fluorescein isothiocyanate (FITC) into the hydrophobic microregions of the cellulose-based aggregations by ultrasound/dialysis method, a series of cellulose-based fluorescent aggregations with different morphologies was obtained.
View Article and Find Full Text PDFPolymers (Basel)
November 2024
Department of Organic and Polymer Materials Chemistry, Tokyo University of Agriculture and Technology, Koganei-shi, Tokyo 184-8588, Japan.
We investigated the crystallization kinetics and morphology evolution of miscible crystalline/crystalline blends of poly(trimethylene terephthalate) (PTT) and poly(ethylene terephthalate) (PET) during isothermal melt crystallization. The integrated light-scattering intensity and the spherulite size increased gradually and then steeply as crystallization progressed in 70/30 PTT/PET at 215 °C, indicating the two-step crystallization behavior. The compact PET spherulite grew in the first step, and the dendritic PTT spherulite grew in the second step, forming the double spherulite consisting of a PET component in the inner region and a PTT one in the outer region.
View Article and Find Full Text PDFTalanta
December 2024
Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, PR China. Electronic address:
As a promising cancer biomarker, microRNA-21 (miRNA-21) has attracted great attention. However, the assay sensitivity of miRNA-21 is highly demanded due to its low abundance. In this work, a highly sensitive sensing platform for miRNA-21 detection was developed based on hybridization chain reaction (HCR) and magnetic beads (MBs)-assisted cascade signal amplification strategy with helical gold nanorods (HGNRs) as dark-field light scattering probes.
View Article and Find Full Text PDFChempluschem
December 2024
Department of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Str. 4-6, 44227, Dortmund, Germany.
The critical micelle concentration is an important property of supramolecular detergents. Two dynamic light scattering approaches have been developed for critical micelle concentration analysis, i. e.
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