Human β-defensin-2(HBD-2) is one of the two major vertebrate antimicrobial peptide families (α and β), which is highly expressed by proinflammatory induction in the lung and exhibit broad-spectrum antimicrobial activity. We observed that IL-22 receptors high expressed on the membrane of A549 cells; HBD-2 mRNA was expressed in a time- and concentration-dependent manners in A549 cells when treated with IL-22; further studies demonstrated that HBD-2 expression was attenuated by AG490, but to JSH-23, inhibitors of p-STAT3 DNA binding and NF-κB/p65 subunit nuclear translocation, respectively. These results support that IL-22-mediated signalling pathway of HBD-2 gene expression involved STAT3 but not NF-κB in human alveolar epithelium. These findings provide a new insight into how IL-22 may play an important link between innate and adaptive immunity, thereby anti-infection locally in the alveolar epithelium.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10753-014-0083-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease.
J Vis Exp
December 2024
Sanford Consortium for Regenerative Medicine; Sanford Burnham Prebys Medical Discovery Institute; Department of Pediatrics, University of California, San Diego School of Medicine;
Human lung tissue is composed of an interconnected network of epithelium, mesenchyme, endothelium, and immune cells from the upper airway of the nasopharynx to the smallest alveolar sac. Interactions between these cells are crucial in lung development and disease, acting as a barrier against harmful chemicals and pathogens. Current in vitro co-culture models utilize immortalized cell lines with different biological backgrounds, which may not accurately represent the cellular milieu or interactions of the lung.
View Article and Find Full Text PDFDidecyldimethylammonium chloride-induced lung fibrosis may be associated with phospholipidosis.
Toxicol Appl Pharmacol
December 2024
College of Medicine, Graduate School, Kyung Hee University, 02447, Republic of Korea; Division of Cardiology, Department of Internal Medicine, Kyung-Hee University Hospital, Kyung Hee University, 02447, Republic of Korea. Electronic address:
In the current study, we dosed Didecyldimethylammonium chloride (DDAC) in mice by pharyngeal aspiration for 28 days or 90 days (weekly) and tried to elucidate the relationship between lamellar body formation and the lesions. When exposed for 28 days (0, 5, 10, 50, and 100 μg/head), all the mice in the 50 and 100 μg/head groups died since Day 2 after the third dosing (Day 16 after the first dosing). Edema, necrosis of bronchiolar and alveolar epithelium, and fibrinous exudate were observed in the lungs of all the dead mice, and chronic inflammatory lesions were observed in the lung tissues of alive mice.
View Article and Find Full Text PDFVerapamil inhibits ferroptosis in septic acute lung injury by blocking L-type calcium channels.
Biochem Biophys Res Commun
December 2024
Immunology Department of Hebei Medical University, Shijiazhuang, PR China. Electronic address:
Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), result from pulmonary edema and alveolar-capillary barrier disruption due to inflammation, often triggered by conditions like sepsis. Sepsis-induced ALI (SALI) involves extensive damage to vascular endothelium and alveolar epithelium, leading to respiratory failure. Our study explores ferroptosis, an iron-dependent cell death pathway, and calcium dysregulation in SALI.
View Article and Find Full Text PDFModelling SARS-CoV-2 infection in a human alveolus microphysiological system.
Access Microbiol
September 2024
Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
The coronavirus 2019 pandemic has highlighted the importance of physiologically relevant models to assist preclinical research. Here, we describe the adaptation of a human alveolus microphysiological system (MPS) model consisting of primary human alveolar epithelial and lung microvascular endothelial cells to study infection with SARS-CoV-2 at Biosafety Level 3 facility. This infection model recapitulates breathing-like stretch and culture of epithelial cells at the air-liquid interface and resulted in clinically relevant cytopathic effects including cell rounding of alveolar type 2 cells and disruption of the tight junction protein occludin.
View Article and Find Full Text PDF[Localization features of FAP-positive activated stromal cells in fibrosis in patients with new coronavirus infection COVID-19].
Arkh Patol
December 2024
Lomonosov Moscow State University, Medical Research and Educational Institute, Moscow, Russia.
Objective: To evaluate the representation and localization of FAP-positive activated stromal cells depending on the severity of fibrotic changes in tissues of patients with a confirmed diagnosis of COVID-19.
Material And Methods: 20 autopsy observations of patients who died from COVID-19 were studied. Immunohistochemical studies were performed using antibodies to CD90, FAP and aSMA and a dual imaging system.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!