Using a television-based vital microscopy method and immunohystochemical analysis, we have assessed the effect of intracerebral transplantation of syngeneic mesenchymal stem cells (MSC) on the brain cortex structure and the microcirculation in the pia mater of old rats. Using "open field" system, we have studied the effect of MSC transplantation on position-finding and discovery behavior of older animals. We have found that density of microvascular network of the pia mater increased ca. 1.9-fold in MSC recipients, compared to age-matched intact animals. Density of the arteriolar area of microvascular network of the pia mater increased ca. 2-fold. Reactivity of the newly formed arterioles was nearly equal to that of native microvessels. Intracerebral transplantation procedure itself was traumatic for brain cortex of rats, but it had no effect on the microcirculation in the contralateral hemisphere. Intracerebral transplantation of MSC did not improve locomotor behavi- or and emotional stage of old rats, did not increase their position-finding and discovery activity.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The Potential of Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Regenerative Medicine.
Methods Mol Biol
December 2024
AntiCancer, Inc., San Diego, CA, USA.
Nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells from mouse and human have been shown to differentiate into neurons, glia, keratinocytes, smooth muscle cells, cardiac muscle cells, and melanocytes in vitro. HAP stem cells have promoted the recovery of peripheral nerve and spinal cord injuries in mouse models by differentiating into glial fibrillary acidic protein (GFAP)-positive Schwann cells. HAP stem cells enclosed on polyvinylidene fluoride membranes (PFM) were transplanted into the severed thoracic spinal cord of nude mice.
View Article and Find Full Text PDFPhase I/II trial of intracerebral transplantation of autologous bone marrow stem cells combined with recombinant peptide scaffold for patients with chronic intracerebral haemorrhage: a study protocol.
BMJ Open
December 2024
Department of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Article Synopsis
- Intracerebral hemorrhage (ICH) is a major cause of death and disability globally, with few treatment options, prompting research into the potential of stem cell therapies, specifically using bone marrow stromal cells (BMSCs) combined with scaffolds.
- The RAINBOW-Hx trial aims to evaluate the safety and effectiveness of this treatment in eight patients with chronic ICH, focusing on those with significant neurological deficits for over a year.
- The study has received ethical approval and involves detailed procedures like bone marrow extraction, cell cultivation, and various imaging assessments post-treatment to monitor patient outcomes.
3D biological scaffold delivers Bergenin to reduce neuroinflammation in rats with cerebral hemorrhage.
J Transl Med
October 2024
Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
Background: Intracerebral hemorrhage (ICH) is a severe form of stroke characterized by high incidence and mortality rates. Currently, there is a significant lack of effective treatments aimed at improving clinical outcomes. Our research team has developed a three-dimensional (3D) biological scaffold that incorporates Bergenin, allowing for the sustained release of the compound.
View Article and Find Full Text PDFCytocidal Effects of Interstitial Photodynamic Therapy Using Talaporfin Sodium and a Semiconductor Laser in a Rat Intracerebral Glioma Model.
Biomedicines
September 2024
Department of Neurosurgery, Tokyo Medical University, Tokyo 160-0023, Japan.
This preclinical study was conducted to investigate the efficacy of interstitial PDT (i-PDT) for malignant gliomas arising deep within the brain, which are difficult to remove. C6 glioma cells were implanted into the basal ganglia of rats, and 3 weeks later, the second-generation photosensitizer talaporfin sodium (TPS) was administered intraperitoneally. Ninety minutes after administration, a prototype fine plastic optical fiber was punctured into the tumor tissue, and semiconductor laser light was irradiated into the tumor from a 2-mm cylindrical light-emitting source under various conditions.
View Article and Find Full Text PDFGut microbiota dysbiosis and neurological function recovery after intracerebral hemorrhage: an analysis of clinical samples.
Microbiol Spectr
November 2024
Department of Anesthesiology and Perioperative Medicine, Zhengzhou central Hospital Affiliated To Zhengzhou University, Zhengzhou University, Zhengzhou, Henan Province, China.
Article Synopsis
- The study investigates the microbial community in patients with intracerebral hemorrhage (ICH) and its impact on neurological prognosis, using fecal samples collected at three different time points.
- Results showed significant differences in gut microbiota composition between ICH patients and healthy controls, with specific bacteria linked to patient outcomes.
- The findings suggest that certain gut microbiota changes could be correlated with neurological function, highlighting their potential as independent risk factors for ICH prognosis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!