The avidin-biotin system was adapted in view of the identification and dosage of the Sendai parainfluenza virus and of its antigens, using the method of double antibodies (biotinylated and nonbiotinylated) in ELISA type tests.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Corosolic acid and its derivatives targeting MCCC1 against insulin resistance and their hypoglycemic effect on type 2 diabetic mice.
Eur J Med Chem
February 2025
College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, China. Electronic address:
Corosolic acid (CA), a natural triterpenoid, exhibits various biological activities and is often called as plant-derived insulin due to its significant hypoglycemic effects, making it especially beneficial for individuals with diabetes or high blood glucose levels. However, CA has notable in vitro toxicity, low water solubility, and poor pharmacokinetic properties. To address these limitations, a series of CA derivatives were synthesized, resulting in the identification of derivative H26, which demonstrates a significantly enhanced hypoglycemic effect, reduced toxicity, and improved pharmacokinetic characteristics compared to CA.
View Article and Find Full Text PDFProtocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.
STAR Protoc
December 2024
Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:
Article Synopsis
- * The text outlines a specific protocol for tyramide signal amplification (TSA) immunohistochemistry aimed at detecting Notch signaling components in blood vessels.
- * It provides detailed steps including antigen retrieval, using specific blocking solutions, and a complex involving avidin/biotin-horseradish peroxidase conjugate with tyramide, as well as optimized washing procedures.
An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers.
J Vis Exp
November 2024
Department of Medicine I, University Hospital, LMU Munich; Institute of Surgical Research at the Walter Brendel Centre of Experimental Medicine, University Hospital, LMU Munich; Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer, Ludwig-Maximilians-University Munich; DZHK (German Centre for Cardiovascular Research), Partner site Munich Heart Alliance;
Despite being anucleated cell fragments, platelets are now widely recognized for their multifaceted abilities. Not only do they form blood clots to prevent bleeding after injury, but they also fight infections and maintain vascular integrity during inflammatory diseases. While hemostatic plugs require the collective activation and aggregation of platelets, their role in protecting inflamed blood vessels is performed at the single-cell level.
View Article and Find Full Text PDFModified poly-L-lysine for use as a clearing agent in pretargeted radioimmunotherapy.
EJNMMI Radiopharm Chem
November 2024
Department of Medical Radiation Sciences, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Background: Pretargeted radioimmunotherapy of cancer has the potential to increase tumor specific uptake of activity when compared with conventional radioimmunotherapy. This is especially true in radioimmunotherapy with nuclides that exhibit a relatively short half-life. When administering antibody-based pretargeting molecules systemically, the antibodies often show a relatively slow clearance from the blood.
View Article and Find Full Text PDFA novel mertansine conjugate for acid-reversible targeted drug delivery validated through the Avidin-Nucleic-Acid-NanoASsembly platform.
Nanomedicine
November 2024
Pharmaceutical and Pharmacological Sciences Dept (DSF), University of Padova, Via Marzolo, 5. 35131 Padova, Italy. Electronic address:
In targeted cancer therapy, antibody-drug-conjugates using mertansine (DM1)-based cytotoxic compounds rely on covalent bonds for drug conjugation. Consequently, the cytotoxic DM1 derivative released upon their proteolytic digestion is up to 1000-fold less potent than DM1 and lacks a bystander effect. To overcome these limitations, we developed a DM1 derivative (keto-DM1) suitable for bioconjugation through an acid-reversible hydrazone bond.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!