AI Article Synopsis
- Scientists can turn regular body cells into special stem cells by using four important factors called Oct4, Klf4, c-Myc, and Sox2 (OKMS).
- They studied how the cells change during this process using a method called mass spectrometry to look closely at proteins, which are important parts of cells.
- The researchers found two main times when big changes happen in the proteins, which help the cells transform into different types of cells with special abilities.
Article Abstract
The ectopic expression of Oct4, Klf4, c-Myc and Sox2 (OKMS) transcription factors allows reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The reprogramming process, which involves a complex network of molecular events, is not yet fully characterized. Here we perform a quantitative mass spectrometry-based analysis to probe in-depth dynamic proteome changes during somatic cell reprogramming. Our data reveal defined waves of proteome resetting, with the first wave occurring 48 h after the activation of the reprogramming transgenes and involving specific biological processes linked to the c-Myc transcriptional network. A second wave of proteome reorganization occurs in a later stage of reprogramming, where we characterize the proteome of two distinct pluripotent cellular populations. In addition, the overlay of our proteome resource with parallel generated -omics data is explored to identify post-transcriptionally regulated proteins involved in key steps during reprogramming.
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| http://dx.doi.org/10.1038/ncomms6613 | DOI Listing |
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