In studying the proteolytic activity and ESR spectra of gamma-irradiated samples of trypsin immobilized at an inoculated copolymer of polypropylene with polyacrylic acid it was established that the carrier of a modified polypropylene increases the radioresistance of trypsin immobilized on it.
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Identification of a dengue 2 virus envelope protein receptor in critical for viral midgut infection.
Proc Natl Acad Sci U S A
November 2024
Laboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, NIH, Rockville, MD 20852.
The establishment of a productive dengue virus (DENV) infection in the midgut epithelial cells of is critical for the viral transmission cycle. The hypothesis that DENV virions interact directly with specific mosquito midgut proteins was explored. We found that DENV serotype 2 (DENV2) pretreated with trypsin interacted with a single 31 kDa protein, identified as AAEL011180 by protein mass spectrometry.
View Article and Find Full Text PDFAspergillus flavipes L-methionine γ-lyase-β-cyclodextrin conjugates with improved stability, catalytic efficiency and anticancer activity.
Sci Rep
November 2024
Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
Aspergillus flavipes L-methionine γ-lyase (MGL) has been authenticated as a powerful anticancer agent towards various solid tumors, however, the catalytic efficiency and stability of this enzyme remains the main challenge for its further in vivo applications. Thus, the objective of this study was to enhance the catalytic efficiency, structural stability of A. flavipes MGL, in addition to boost their anticancer activity, via conjugation with β-cyclodextrin.
View Article and Find Full Text PDFDetection of factor Xa activity using giant magnetoresistive biosensors.
Anal Chim Acta
December 2024
Department of Mechanical and Biomedical Engineering, Ewha Womans University, Seoul, 03760, Republic of Korea; Graduate Program in Smart Factory, Ewha Womans University, Seoul, 03760, Republic of Korea. Electronic address:
Background: As anticoagulants are widely used to treat patients with atrial fibrillation (AF) and other thrombotic conditions, it is necessary for physicians to determine whether the medication has been taken in emergencies. Among many anticoagulants, rivaroxaban has attracted attention due to its safety and convenience. Since rivaroxaban inhibits activated coagulation factor X (factor Xa), measuring factor Xa activity can determine the presence of rivaroxaban.
View Article and Find Full Text PDFStability of immobilized L-arginine deiminase from Penicillium chrysogenum and evaluation of its anticancer activity.
Sci Rep
November 2024
Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
The aim of the present work was to immobilize L-arginine deiminase on suitable supports such as chitosan, alginate, and silica gel to study its stability. Additionally, the study aims to investigate the anticancer effects of the free purified enzyme on hepatocellular carcinoma (Hep-G2) and breast cancer (MCF-7) cell lines. L-arginine deiminase (ADI: EC 3.
View Article and Find Full Text PDFProteome characterization of extracellular vesicles from human milk: Uncovering the surfaceome by a lipid-based protein immobilization technology.
J Extracell Biol
November 2024
Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection Linköping University Linkoping Sweden.
Breast milk is an essential source of nutrition and hydration for the infant. In addition, this highly complex fluid is rich in extracellular vesicles (EVs). Here, we have applied a microfluidic technology, lipid-based protein immobilization (LPI) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) to characterize the proteome of human milk EVs.
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