A method is described for the specific detection of antibody to individual rotavirus serotypes in sera. A competitive enzyme immunoassay (EIA) was developed in which rotavirus serotype-specific monoclonal antibodies against VP7 compete with antibodies in test sera for rotavirus serotype-specific antigen bound to a solid phase. There was an excellent correlation between serotype-specific EIA results and serotype-specific neutralization titres (r = 0.915, P = less than 0.001). The value of this method for rotavirus epidemiology and vaccine trials is discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0166-0934(89)90012-8 | DOI Listing |
Publication Analysis
Top Keywords
rotavirus serotype-specific
12
sera competitive
8
enzyme immunoassay
8
serotype-specific
5
determination rotavirus
4
serotype-specific antibodies
4
antibodies sera
4
competitive enhanced
4
enhanced enzyme
4
immunoassay method
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!