AI Article Synopsis
- The β-ketoester benzyl acetoacetate was successfully reduced to benzyl (S)-3-hydroxybutanoate using various microorganisms, with Hansenula sp. achieving the highest enantiomeric excess (ee) of 97% and 85% conversion in 24 hours.
- After immobilizing the yeast Kluyveromyces marxianus in calcium alginate spheres, it demonstrated improved stability, remaining effective through two reaction cycles.
- This study highlights the potential of specific yeasts in efficiently catalyzing enantioselective reductions in biochemical applications.
Article Abstract
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204978 | PMC |
| http://dx.doi.org/10.1590/s1517-83822014000300023 | DOI Listing |
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