This study sought to develop customized morphology flagging thresholds for canine erythrocyte volume and hemoglobin concentration [Hgb] on the ADVIA 120 hematology analyzer; compare automated morphology flagging with results of microscopic blood smear evaluation; and examine effects of customized thresholds on morphology flagging results. Customized thresholds were determined using data from 52 clinically healthy dogs. Blood smear evaluation and automated morphology flagging results were correlated with mean cell volume (MCV) and cellular hemoglobin concentration mean (CHCM) in 26 dogs. Customized thresholds were applied retroactively to complete blood (cell) count (CBC) data from 5 groups of dogs, including a reference sample group, clinical cases, and animals with experimentally induced iron deficiency anemia. Automated morphology flagging correlated more highly with MCV or CHCM than did blood smear evaluation; correlation with MCV was highest using customized thresholds. Customized morphology flagging thresholds resulted in more sensitive detection of microcytosis, macrocytosis, and hypochromasia than default thresholds.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231806 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Accelerating antiviral drug discovery: early hazard detection with a dual zebrafish and cell culture screen of a 403 compound library.
Arch Toxicol
December 2024
Department of Environmental and Molecular Toxicology, Sinnhuber Aquatic Research Laboratory, Oregon State University, Corvallis, OR, 97333, USA.
The constant emergence of new viral pathogens underscores the need for continually evolving, effective antiviral drugs. A key challenge is identifying compounds that are both efficacious and safe, as many candidates fail during development due to unforeseen toxicity. To address this, the embryonic zebrafish morphology, mortality, and behavior (ZBE) screen and the SYSTEMETRIC® Cell Health Screen (CHS) were employed to evaluate the safety of 403 compounds from the Cayman Antiviral Screening Library.
View Article and Find Full Text PDFFlagging records of Ixodes frontalis (Panzer, 1798) and dermacentor marginatus (Sulzer, 1776) (Acari: Ixodidae), and their first reporting from Coblenz region, Western Germany.
Exp Appl Acarol
December 2024
Department for Microbiology, Bundeswehr Central Hospital, Coblenz, Germany.
Contrawise to mosquitoes, there is no country-wide long-term surveillance of ticks in Germany, leading to large gaps in coverage for distribution data. Here we report on results from two consecutive tick studies in northern Rhineland-Palatinate, western Germany, conducted in 2022 and 2023. In 2022, a new focus of the ornate sheep tick Dermacentor marginatus was detected at a military training area east of Coblenz ("Schmidtenhöhe") which is partially managed as a nature reserve where old races of cattle and horses are grazing.
View Article and Find Full Text PDFHeart Failure with Preserved Ejection Fraction and Cardiac Amyloidosis in the Aging Heart.
Int J Mol Sci
October 2024
Geriatric Clinic, SS. Annunziata Hospital, 66100 Chieti, Italy.
Article Synopsis
- - Heart Failure with Preserved Ejection Fraction (HFpEF) affects a significant portion of the population, particularly the elderly, leading to high hospitalization rates (70-80%) and substantial mortality (40-50% over five years).
- - The condition is characterized by increased stiffness in heart muscle cells, causing diastolic dysfunction and elevated filling pressures, with cardiac amyloidosis (CA) being a notable cause that leads to similar symptoms.
- - There’s an urgent need to recognize and screen for transthyretin amyloidosis (ATTR) in older HFpEF patients, as early diagnosis can greatly improve treatment outcomes.
Anorexia nervosa in children and adolescents: an early detection of risk factors.
Ital J Pediatr
October 2024
The Italian Pediatric Society Adolescent Study Group, The Italian Pediatric Society, Rome, Italy.
The incidence of eating disorders in children, mainly of anorexia nervosa, is dramatically increased in the last years. A timely identification of the disease is associated with higher rates of recovery. Aim of the work is to underline signs and symptoms that can be used to an early detection of anorexia nervosa in the pediatric age.
View Article and Find Full Text PDFCirculating proteins linked to apoptosis processes and fast development of end-stage kidney disease in diabetes.
JCI Insight
October 2024
Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA.
Many circulating proteins are associated with risk of ESKD, but their source and the biological pathways/disease processes they represent are unclear. Using OLINK proteomics platform, concentrations of 455 proteins were measured in plasma specimens obtained at baseline from 399 individuals with diabetes. Elevated concentrations of 46 circulating proteins were associated (P < 1 × 10-5) with development of ESKD (n = 143) during 7-15 years of follow-up.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!