AI Article Synopsis
- Researchers screened eight types of halophilic bacteria from Kovalam solar salt works in India for their ability to produce biosurfactants.
- Kocuria sp., Kurthia sp., and Halococcus sp. showed positive biosurfactant activity, with Kocuria sp. exhibiting the strongest emulsification of various oils.
- The biosurfactant from Kocuria marina BS-15 was purified and characterized, identifying it as a lipopeptide with compounds like Nonanoic acid and Cyclopropane using techniques such as TLC, FTIR, and GC-MS.
Article Abstract
Biosurfactant screening was made among the eight halophilic bacterial genera isolated from Kovalam solar salt works in Kanyakumari of India. After initial screening, Kocuria sp. (Km), Kurthia sp. (Ku) and Halococcus sp. (Hc) were found to have positive biosurfactant activity. Biosurfactant derived from Kocuria sp. emulsified more than 50% of the crude oil, coconut oil, sunflower oil, olive oil and kerosene when compared to the other strains. Further, Kocuria marina BS-15 derived biosurfactant was purified and characterized by TLC, FTIR and GC-MS analysis. The TLC analysis revealed that, the purified biosurfactants belong to the lipopeptide group. The IR spectrum results revealed that functional groups are R2C = N = N, alkenes and N-H. The GC-MS analysis confirmed the compound as Nonanoic acid and Cyclopropane with the retention time of 12.78 and 24.65, respectively.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250494 | PMC |
| http://dx.doi.org/10.1016/j.sjbs.2014.01.001 | DOI Listing |
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