Ligand-mediated prostate cancer (PCa)-targeting gene delivery is one of the focuses of research in recent years. Our previous study reported the successful preparation of aptamer-modified nanoparticles (APT-NPs) in our laboratory and demonstrated their PCa-targeting ability in vitro. However, the mechanism underlying this PCa-targeting effect and their anticancer ability in vivo have not yet been elucidated. The objective of this study was to assess the feasibility of using APT-NPs to deliver micro RNA (miRNA) systemically to PCa cells, to testify their tumor-targeting efficiency, and to observe their biodistribution after systemic administration to a xenograft mouse model of PCa. In addition, the effect of APT depletion and endocytosis inhibitors on cellular uptake was also evaluated quantitatively in LNCaP cells to explore the internalization mechanism of APT-NPs. Finally, blood chemistry, and renal and liver function parameters were measured in the xenograft mouse model of PCa to see whether APT-NPs had any demonstrable toxicity in mice in vivo. The results showed that APT-NPs prolonged the survival duration of the PCa tumor-bearing mice as compared with the unmodified NPs. In addition, they had a potential PCa-targeting effect in vivo. In conclusion, this research provides a prototype for the safe and efficient delivery of miRNA expression vectors to PCa cells, which may prove useful for preclinical and clinical studies on the treatment of PCa.
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http://dx.doi.org/10.2147/IJN.S71101 | DOI Listing |
J Mater Chem B
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Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, 410012, China.
Combination of immunotherapy and photothermal therapy (PTT) provides a promising therapeutic performance for tumors. However, it still faces negative feedback from suppressive factors such as adenosine. Herein, we developed a new nanodrug that can combine adenosine blockade and ferroptosis to promote the photoimmunotherapy of triple negative breast cancer (TNBC).
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February 2025
Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, PR China. Electronic address:
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April 2025
College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China. Electronic address:
Glutathione (GSH) is a key biomarker closely associated with cancer, and its content varies greatly between normal cells and cancer cells. However, intracellular detection of GSH was challenging because existing probes not only have a long detection time but also have fluorescence in the blue-green region that overlaps with the biological matrix's spontaneous fluorescence, thus affecting the detection accuracy. Therefore, a new red fluorescent nano-probe was needed to rapidly and accurately detected GSH within the biological matrix.
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Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Nursing, Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China. Electronic address:
Designing and inventing synergistic emerging antimicrobial strategies is critical for mitigating potential resistance to conventional antibiotics. This task is challenging because these antimicrobial agents should need to eliminate bacteria, slow oxidative stress in wounds, and be safe and nontoxic. Here, we report a highly safe antimicrobial nanocatalyst for bacterial scavenging through aptamer-synergistic multienzyme activity.
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February 2025
School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122, China. Electronic address:
Mucin1 (MUC1) is a cell surface transmembrane protein overexpressed in multiple types of tumor cells, which is generally considered as a tumor-associated biomarker. Thus, quantifying and imaging of MUC1 in tumor cells is of great significance for the diagnosis and biological therapy of tumors. Herein, a fluorescence (FL) and surface-enhanced Raman scattering (SERS) dual-mode biosensor was developed for sensitive detection and imaging of MUC1 in living cells.
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