Background: Krüppel-like factor 4 (KLF4) is a transcription factor that regulates a diverse array of cellular processes, including development, differentiation, proliferation, and apoptosis. Although its function in keratinocytes has been widely studied, its exact role in psoriasis has not been elucidated.
Objective: We designed this study to investigate epidermal expression levels of KLF4 and the change in KLF4 expression after treatment in patients with psoriasis.
Methods: We compared the expression levels of KLF4 in the basal, suprabasal, and superficial epidermal layers, in psoriatic lesional, non-lesional, and normal skin, using an immunoreactivity intensity distribution index (IRIDI). In addition, we measured the change in KLF4 expression on the basis of the IRIDI and by reverse transcription polymerase chain reaction (RT-PCR) analysis after treatment.
Results: The combined IRIDI scores in psoriatic lesional skin were significantly higher than the scores in both non-lesional and normal skin. The psoriatic epidermis, particularly the suprabasal layer, showed a significantly increased IRIDI score compared to that of non-lesional and normal skin, which was significantly decreased after treatment. RT-PCR analysis exhibited a slight increase in KLF4 mRNA expression level after treatment; however, this increase was not significant.
Conclusion: These data indicate that KLF4 could regulate epidermal proliferation and differentiation. Moreover, we believe that KLF4 may play an important role in the physiological reaction to counteract abnormal differentiation and proliferation of keratinocytes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252662 | PMC |
| http://dx.doi.org/10.5021/ad.2014.26.6.675 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SIRT6 knockdown alleviates keratinocyte hyperproliferation and inflammation in psoriasis via modulating acetylation of FOXO1.
Int Immunopharmacol
December 2024
Department of Dermatology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
The Sirtuins family (SIRT) has been implicated in numerous diseases, including psoriasis.However, the precise role of SIRT6 in psoriasis remains unclear. The analysis of publicly available RNA-seq data from GEO profiles showed that SIRT6 expression levels was significantly elevated in the lesional skins from patients with psoriasis, as compared to the non-lesional skins or the skins from normal healthy donors.
View Article and Find Full Text PDFAlterations in white matter integrity and correlations with clinical characteristics in children with non-lesional temporal lobe epilepsy.
Seizure
December 2024
Department of Radiology, Children's Hospital of Fudan University, No 399 Wanyuan Road, Shanghai 201102, PR China. Electronic address:
Purpose: To complement the current research on altered white matter integrity in children with non-lesional temporal lobe epilepsy (NL-TLE), especially the correlation between diffusion metrics and clinical characteristics, so as to provide imaging evidence for clinical practice.
Methods: Children with temporal lobe epilepsy and no lesions on magnetic resonance imaging (MRI) were retrospectively collected from 2016.01.
Lateralizing Value of Artificial Intelligence-Based Segmentation Software in MRI-Negative Focal Epilepsy.
J Epilepsy Res
December 2024
Department of Neurology, Seoul National University College of Medicine, Seoul, Korea.
Background And Purpose: The magnetic resonance images (MRIs) ability of lesion detection in epilepsy is crucial for a diagnosis and surgical outcome. Using automated artificial intelligence (AI)-based tools for measuring cortical thickness and brain volume originally developed for dementia, we aimed to identify whether it could lateralize epilepsy with normal MRIs.
Methods: Non-lesional 3-Tesla MRIs of 428 patients diagnosed with focal epilepsy, based on semiology and electroencephalography findings, were analyzed.
Profiling of Toll-like Receptors and Related Signaling Mediators in the Pathogenesis of Morphea.
Dermatol Pract Concept
October 2024
Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Türkiye.
Introduction: Morphea, also known as localized scleroderma, is a rare fibrosing inflammatory disease of unknown pathogenesis.
Objectives: Although the genetic basis for morphea is important, reports on the evaluation of Toll-like receptors (TLR) in this disease is quite limited. We aimed to evaluate TLR expression levels and serum IL-6, IL-17A, TGF-β1, FGF, and VEGF levels in patients with morphea and compare these results with healthy controls.
Secukinumab Reduces Psoriasis-associated Pruritus and Regenerates the Cutaneous Nerve Architecture: Results from PSORITUS a Doubleblind, Placebo-controlled, Randomized Withdrawal Phase IIIb Study.
Acta Derm Venereol
November 2024
1Department of Dermatology, University Hospital Münster, Münster, Germany; Center for Chronic Pruritus, University Hospital Münster, Münster, Germany.
The occurrence of pruritus in psoriasis was previously underestimated but is a significant burden. Secukinumab (SEC), a monoclonal anti-interleukin-17A antibody, efficiently controls signs of psoriasis, but the effect on pruritus and cutaneous neuroanatomy remained unknown. The primary objective of this study (NCT02362789) was to evaluate the superiority of SEC treatment vs placebo on pruritus intensity (visual analogue scale; VAS).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!