AI Article Synopsis
- Two types of ELISA tests (competitive and sandwich) were developed to specifically measure human leukocyte collagenase and gelatinase levels.
- The competitive assay has a detection limit of 0.5 ng for collagenase and 0.05 ng for gelatinase, while the sandwich ELISA can detect as low as 0.05 ng and 0.02 ng for these enzymes, respectively.
- In plasma samples, collagenase levels ranged from 0 to 50 micrograms/l, and gelatinase levels ranged from 27 to 94 micrograms/l, with no cross-reactivity observed between the two enzymes.
Article Abstract
A competitive and a sandwich enzyme linked immunosorbent assay (ELISA) were developed for human leukocyte collagenase and gelatinase. The competitive assay could detect 0.5 ng collagenase and 0.05 ng gelatinase. The detection limit of the sandwich ELISA was 0.05 ng for collagenase and 0.02 ng for gelatinase. No cross reactivity between human leukocyte collagenase and gelatinase was detected. The sandwich ELISA was used to determine plasma levels of these enzymes. The 90% range for collagenase was between 0 and 50 micrograms/l; the 90% range for gelatinase was between 27 and 94 micrograms/l.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1515/cclm.1989.27.6.351 | DOI Listing |
Publication Analysis
Top Keywords
human leukocyte
12
leukocyte collagenase
12
collagenase gelatinase
12
enzyme linked
8
linked immunosorbent
8
gelatinase competitive
8
sandwich elisa
8
90% range
8
collagenase
6
gelatinase
6
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!