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Implantation of an ultrafiltration device in the ileum and spiral colon of steers to continuously collect intestinal fluid. | LitMetric

Implantation of an ultrafiltration device in the ileum and spiral colon of steers to continuously collect intestinal fluid.

Res Vet Sci

Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States. Electronic address:

Published: December 2014

AI Article Synopsis

Article Abstract

Collection of fluid from the lumen of the gastrointestinal tract is commonly necessary for research projects, but presents challenges including intestinal motility and potential for leakage of intestinal contents. In this study, ultrafiltration collection devices were surgically implanted in the ileum and spiral colon of 12 steers for repeated collection of intestinal fluid over 48 hours. There were no significant complications associated with surgery or during the post-operative period, nor were there any significant pathologic changes found at necropsy 3 or 4 days post-surgery. Over 48 hours, we obtained 88% of the desired 212 samples. Only two devices failed to routinely collect samples. Use of ultrafiltration probes is a novel, consistent and humane method to repeatedly sample the gastrointestinal contents.

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Source
http://dx.doi.org/10.1016/j.rvsc.2014.10.012DOI Listing

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