Zinc is an essential trace element for human growth and development. In this work, zinc-chelating peptides from rapeseed protein hydrolysates produced with alcalase were investigated by affinity chromatography with immobilized zinc and Sephadex G-25 gel filtration. Four small peptides, namely, Ala-Arg, Asn-Ser-Met (NSM), Gly-Lys-Arg, and Glu-Pro-Ser-His, were obtained and identified by reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry. The zinc-chelating ability of the four peptides was further validated by inductively coupled plasma atomic emission spectrometry (ICP-AES). NSM was found to exhibit the highest zinc-chelating rate, which was better than that of reduced glutathione. We speculated that the Asn residue at the amino-terminus might facilitate this zinc-chelating ability. Therefore, utilizing small peptides from rapeseed protein as novel carriers for zinc supplement was feasible.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.foodchem.2014.10.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Improving flavor of strong fragrant rapeseed oils by supplementing commercial peptides and sugars.
Food Chem X
December 2024
Jiangsu Hefeng Grain and Oil Industry Co., Ltd., Yancheng 212002, PR China.
The strong fragrant rapeseed oils (SFROs) attract an increasing consumers' preference due to their strong flavor and attractive appearance. With the accumulated knowledge of flavor formation pathways during producing SFROs, the present study proposed a novel method to enhance SFRO's flavor by directly adding the reducing sugars (glucose and xylose) and commercial peptides during roasting process. Results indicated that supplementation of 3 % rapeseed peptide, 1 % glucose and 0.
View Article and Find Full Text PDFMechanistic study of SCOOPs recognition by MIK2-BAK1 complex reveals the role of N-glycans in plant ligand-receptor-coreceptor complex formation.
Nat Plants
December 2024
National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Ligand-induced receptor and co-receptor heterodimerization is a common mechanism in receptor kinase (RK) signalling activation. SERINE-RICH ENDOGENOUS PEPTIDEs (SCOOPs) mediate the complex formation of Arabidopsis RK MIK2 and co-receptor BAK1, triggering immune responses. Through structural, biochemical and genetic analyses, we demonstrate that SCOOPs use their SxS motif and adjacent residues to bind MIK2 and the carboxy-terminal GGR residues to link MIK2 to BAK1.
View Article and Find Full Text PDFStudy of the in vitro digestibility of oilseed protein concentrates compared to isolates for food applications.
Food Chem
February 2025
Laboratoire Réactions et Génie des Procédés, Université de Lorraine, CNRS, LRGP, F-54000 Nancy, France.
The research has primarily focused on isolates (>90 % protein) when studying oilseed protein products, but there is a growing interest in concentrates (65-90 % protein) due to their industrial viability and lower environmental impact. This study aimed to compare the in vitro digestibility of rapeseed and sunflower protein concentrates with isolates. Simulated digestion was conducted, and the resulting samples were analyzed using a size-exclusion chromatography approach.
View Article and Find Full Text PDFBrassica rapa L. (Tibetan Turnip) polysaccharide improves the immune function and regulates intestinal microbiota in immunosuppressive mice.
J Food Sci
December 2024
College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, China.
Article Synopsis
- Active polysaccharides were extracted from Brassica rapa L. (BRP), with structural characterization indicating it is an acidic heteropolysaccharide composed primarily of Ara, GalA, and GlcA.
- The study found that BRP effectively improved immune functions in cyclophosphamide-induced immunosuppressed mice by boosting thymus and spleen indices, enhancing immune cell activity, and increasing antioxidant levels.
- BRP also elevated serum cytokines and immunoglobulins, influenced key immune signaling pathways, and modified the composition of intestinal microbiota, suggesting its potential as a natural immunomodulator.
Amelioration of Type 2 Diabetes Mellitus Using Rapeseed ()-Derived Peptides through Stimulating Calcium-Sensing Receptor: Effects on Glucagon-Like Peptide-1 Secretion and Hepatic Lipid Metabolism.
J Agric Food Chem
October 2024
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, People's Republic of China.
The potential of rapeseed-derived peptides (RDPs) in the amelioration of type 2 diabetes mellitus (T2DM) has been hypothesized. However, the mechanisms of the intestinal endocrine hormones activated by RDPs have not been fully understood. This study aimed to explore the amelioration of T2DM and associated hepatic lipid metabolism disorders using RDPs by affecting glucagon-like peptide-1 (GLP-1) secretion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!