Water Res
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Published: February 2015
The purpose of this study was to explore how fast the thermophilic anaerobic microbial community could be established during the one-step startup of thermophilic anaerobic digestion from a mesophilic digester. Stable thermophilic anaerobic digestion was achieved within 20 days from a mesophilic digester treating sewage sludge by adopting the one-step startup strategy. The succession of archaeal and bacterial populations over a period of 60 days after the temperature increment was followed by using 454-pyrosequencing and quantitative PCR. After the increase of temperature, thermophilic methanogenic community was established within 11 days, which was characterized by the fast colonization of Methanosarcina thermophila and two hydrogenotrophic methanogens (Methanothermobacter spp. and Methanoculleus spp.). At the same time, the bacterial community was dominated by Fervidobacterium, whose relative abundance rapidly increased from 0 to 28.52 % in 18 days, followed by other potential thermophilic genera, such as Clostridium, Coprothermobacter, Anaerobaculum and EM3. The above result demonstrated that the one-step startup strategy could allow the rapid establishment of the thermophilic anaerobic microbial community.
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http://dx.doi.org/10.1016/j.watres.2014.11.001 | DOI Listing |
Appl Environ Microbiol
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Centro de Engenharia Genética e Biologia Molecular (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil.
Unlabelled: is an anaerobic and thermophilic bacterium that has been genetically engineered for ethanol production at very high yields. However, the underlying reactions responsible for electron flow, redox equilibrium, and how they relate to ethanol production in this microbe are not fully elucidated. Therefore, we performed a series of genetic manipulations to investigate the contribution of hydrogenase genes to high ethanol yield, generating evidence for the importance of hydrogen-reacting enzymes in ethanol production.
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Section II: Electrobiotechnology, Institute of Process Engineering in Life Science, Karlsruhe Institute of Technology, 76131, Karlsruhe, Germany.
Background: Parageobacillus thermoglucosidasius is a facultatively anaerobic thermophile that is able to produce hydrogen (H) gas from the oxidation of carbon monoxide through the water-gas shift reaction when grown under anaerobic conditions. The water-gas shift (WGS) reaction is driven by a carbon monoxide dehydrogenase-hydrogenase enzyme complex. Previous experiments exploring hydrogenogenesis with P.
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BioEngine Research Team on Green Process Engineering and Biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine, Québec, Québec, Canada; CentrEau, Centre de recherche sur l'eau, Université Laval, 1065 Avenue de la Médecine, Québec, QC, G1V 0A6, Canada. Electronic address:
Despite advances in anaerobic digestion (AD), full-scale implementation faces significant challenges, particularly during the start-up phase, where inoculum selection is crucial. This study examines the impact of inoculum choice on the operational and economic performance of thermophilic digesters during the start-up phase. Methanogenic reactors R3 and R4 were inoculated with digested sludge (DiS) and diluted sewage sludge (DSS), respectively, and fed with hydrolyzed source-sorted organic fraction of municipal solid waste (SS-OFMSW) and thickened sewage sludge, which were processed in R1 and R2, serving as acidogenic reactors.
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Agricultural Biosystems Engineering Group, Department of Plant Sciences, Wageningen University and Research, P.O. Box 16, 6700 AA, Wageningen, the Netherlands.
Managing dairy excreta as slurry can result in significant emissions of ammonia (NH) and greenhouse gases (GHGs) during storage and thereafter. Additionally, slurry often has an imbalanced nitrogen (N) to phosphorus (P) ratio for crop fertilization. While various treatments exist to address emissions and nutrient imbalances, each has trade-offs that can result in pollution swapping.
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January 2025
BioEngine Research team on green process engineering and biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine Québec (Québec), Canada; CentrEau, Centre de recherche sur l'eau, Université Laval, 1065 Avenue de la Médecine, Québec, QC, G1V 0A6, Canada. Electronic address:
The role of inoculum in initiating anaerobic digestion (AD), and accelerating the start-up of anaerobic digesters has been well-documented. However, the effect of aligning the origin temperature of the inoculum with the operational temperature of the new digester remains underexplored. This study investigates how the origin temperature and characteristics of the inoculum affect the kinetics and biodegradability of sewage sludge (SS) and microcrystalline cellulose (MCC) under mesophilic and thermophilic conditions.
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