AI Article Synopsis
- * Researchers created polylactic-co-glycolic acid nanospheres filled with plasmids to deliver green fluorescence protein and miRNA targeting the transforming growth factor-β1 gene.
- * The study found that these nanosphere/plasmid combinations effectively reduced transforming growth factor-β1 in healing chicken tendons without causing inflammation or tissue damage.
Article Abstract
Background Aims: Sustained gene delivery could be particularly necessary in modulating gene expression in healing intrasynovial tendons and may be a promising approach for preventing adhesions.
Methods: Polylactic-co-glycolic acid nanospheres were prepared and were then incorporated with plasmids expressing enhanced green fluorescence protein and miRNA for inhibiting the transforming growth factor-β1 gene expression.
Results: We demonstrated that cultured tenocytes could be effectively transfected by means of nanosphere/plasmid. The expression of transforming growth factor-β1 is significantly downregulated in healing chicken flexor tendon treated with nanosphere/plasmid. Histology analysis did not demonstrate any significant inflammation or necrosis in tendons injected with nanosphere/plasmid after surgery.
Conclusions: Nanosphere/plasmid may be a promising non-viral vector for gene therapy of the injured flexor tendon.
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| http://dx.doi.org/10.1016/j.jcyt.2014.09.004 | DOI Listing |
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