AI Article Synopsis
- Ovarian follicular atresia is a process where certain cells in the ovary, called granulosa cells, die, but scientists aren't sure why this happens.
- The study found that a protein called SIRT1 is involved in this process, showing that higher levels of SIRT1 are linked to the death of granulosa cells.
- When a substance called resveratrol, which boosts SIRT1, was tested, it was seen to increase both SIRT1 levels and the death rate of granulosa cells, suggesting SIRT1 plays an important role in this cell death in pig ovaries.
Article Abstract
Ovarian follicular atresia is characterized by granulosa cell apoptosis, however, the exact mechanism is still unclear. Sirtuin 1 (SIRT1) is a NAD(+)-dependent deacetylase, which is associated with apoptosis in several of cell types, but its exact role in ovarian granulosa cell apoptosis is not clearly defined. In present study, we identified the involvement of SIRT1 in the process of follicle degeneration, which is known as "follicular atresia", both from in vivo models and cell culture data. The results of immunohistochemistry showed that SIRT1 was widely detected in non-apoptotic granulosa cells of follicles, but significantly decreased during the process of granulosa cell apoptosis. Quantitative real-time PCR and Western blot analysis showed that the expression levels of SIRT1 mRNA and protein were increased (P<0.05) during follicular atresia. In order to provide more evidences elucidating the roles of SIRT1 during the process of follicular atresia, granulosa cells were cultured in vitro with resveratrol which acts as a potent activator of SIRT1. Results showed that resveratrol caused a dose-dependent increase in both SIRT1 mRNA and protein levels. Meanwhile, apoptotic rate of granulosa cell was increased (P<0.01) in a dose-dependent manner. Additionally, resveratrol significantly increased the expression levels of Caspase-3 (P<0.01) and Bax mRNA (P<0.01), while Bcl-2 mRNA level was significantly decreased (P<0.01). Thus, our results suggest that SIRT1 may play important roles in the regulation of granulosa cell apoptosis during follicular atresia in porcine ovary.
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| http://dx.doi.org/10.1016/j.anireprosci.2014.10.002 | DOI Listing |
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