AI Article Synopsis
- A new colorimetric method has been developed to measure inorganic pyrophosphate (PPi) in the presence of orthophosphate.
- The method works by forming complexes with molybdate, which are then extracted using organic solvents.
- It offers a sensitivity range of 5 to 120 nmol of PPi per 2.0-ml sample.
Article Abstract
A sensitive colorimetric method for the assay of inorganic pyrophosphate with excess of orthophosphate is described. The principle of this method lies in the formation of phosphomolybdate and PPi-molybdate complexes with subsequent extraction of the phosphomolybdate complex by organic solvents and reduction of the PPi-molybdate complex by dithiothreitol and Eikonogen. The sensitivity of the method was from 5 to 120 nmol of PPi in a 2.0-ml sample.
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| http://dx.doi.org/10.1016/0003-2697(89)90329-1 | DOI Listing |
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