AI Article Synopsis
- NSAIDs like indomethacin are commonly used for injuries and post-surgery, but high doses can inhibit the growth and collagen formation of human tenocytes (tendon cells).
- Lactoferrin, an anabolic agent, promotes tenocyte survival and collagen production, especially in low serum conditions.
- When combined with indomethacin, lactoferrin can partially counteract the negative effects of the NSAID on tenocyte growth and health, indicating its potential as a supportive treatment.
Article Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in patients with injuries and inflammation of tendon and ligament, and as post-surgical analgesics. The aim of this study is to investigate the effect of indomethacin, a classic NSAID and its combinational effect with an anabolic agent of skeletal tissue, lactoferrin, on the proliferation and collagen formation of human tenocytes in vitro. A factorial experimental design was employed to study the dose-dependent effect of the combination of indomethacin and lactoferrin. The results showed that indomethacin at high concentration (100 μM) inhibited human tenocyte proliferation in culture medium with 1-10% fetal bovine serum (FBS) in vitro. Also, high dose of indomethacin inhibited the collagen formation of human tenocytes in 1% FBS culture medium. Lactoferrin at 50-100 μg/ml promoted human tenocyte survival in serum-free culture medium and enhanced proliferation and collagen synthesis of human tenocytes in 1% FBS culture medium. When 50-100 μg/ml lactoferrin was used in combination with 100-200 μM indomethacin, it partially rescued the inhibitory effects of indomethacin on human tenocyte proliferation, viability and collagen formation. To our knowledge, it is the first evidence that lactoferrin is anabolic to human tenocytes in vitro and reverses potential inhibitory effects of NSAIDs on human tenocytes.
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| http://dx.doi.org/10.1016/j.bbrc.2014.10.061 | DOI Listing |
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