The transplantation of coral fragments is one of methods that restore coral communities. To form coral colonies, the fragmented corals initiated skeletal extension from the cut-edge of fragment then success the settlement. In order to understand the molecular events underlying fragment adhesion and settlement, we constructed a normalized cDNA library and generated and annotated expressed sequence tags (ESTs) from the fragmented adult polyps of soft coral Sinularia notanda. We generated 3251 high-quality ESTs with an average length of 580 bp and the EST cluster and assembly analyses produced 2796 unigenes, including 2487 singletons and 309 contigs. Of the known genes, 55 genes were sel ected to be involved in polyp fragment adhesion and settlement based on Gene Ontology (GO) classification. Notably, two EST clones were identified to show homology with galaxin gene which was demonstrated as coral specific calcifying protein of organic matrix. These EST sequences can provide utility as molecular markers in molecular and genetic studies of S. notanda and other soft coral.
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http://dx.doi.org/10.1016/j.margen.2014.10.008 | DOI Listing |
Biofouling
January 2025
The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
Nat Prod Res
November 2024
Department of Chemistry, Faculty of Science, Damietta University, New Damietta, Egypt.
Mar Biotechnol (NY)
November 2024
Institute of Fisheries Science, National Taiwan University, Taipei, 10617, Taiwan.
Octocorals, vital components of reef ecosystems, inhabit various marine environments across diverse climate zones, spanning from tropical shallows to frigid deep-sea regions. Certain octocoral species, notably Lobophytum and Sinularia, are particularly intriguing due to their production of diverse metabolites, warranting continuous investigation. Although octocorals played the roles in coral ecosystems, the studies are rare in comparison to scleractinian corals, especially in transcriptomic and genomic data.
View Article and Find Full Text PDFMar Drugs
September 2024
Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
The chemical investigation of the South China Sea soft coral has resulted in the isolation of seven new terpenoids, including two new meroterpenoids, namely sinudenoids F-G (-), and five new cembranes, namely sinudenoids H-L (-). Their structures and absolute configurations were elucidated based on extensive analyses of spectroscopic data, single-crystal X-ray diffraction, comparison with the literature data, and quantum chemical calculations. Among them, sinudenoid F () and sinudenoid G () are rare meroterpenoids featuring a methyl benzoate core.
View Article and Find Full Text PDFJ Chromatogr A
November 2024
School of Medicine, Shanghai University, 99 Shangda Road, BaoShan District, Shanghai 200444, PR China; Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264117, PR China. Electronic address:
Eleven new macrocyclic casbane-type diterpenes, namely sinueracasbanones E-O (1-11), have been isolated from the South China Sea soft coral Sinularia erecta using combined column chromatography. The structures of new compounds were elucidated by extensive spectroscopic data analysis, the modified Mosher's method, X-ray diffraction analysis and quantum mechanical calculation methods. In bioassay, compounds 2, 5, 10 and 11 displayed interesting anti-inflammatory activities by the inhibition of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α protein release with half maximal inhibitory concentration (IC) values of 15.
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