The role of Ca(2+)-permeable AMPA-receptors (CP-AMPARs) in the induction of paired-pulse depression (PPD) at glutamatergic nociceptive sensory synapses was examined in co-culture of rat's dorsal root ganglion and dorsal horn neurons. CP-AMPARs make a considerable contribution to excitatory postsynaptic currents recorded in DH neurons following action potential generation in nociceptive DRG neurons. Activation of CP-AMPARs during fast synaptic transmission induces strong PPD (with a 200 ms inter-pulse interval). Blockage of CP-AMPARs with Naspm (100 μM) results in most cases in a significant reduction of the PPD magnitude, also reversing to paired-pulse facilitation in some cases. However, loading of postsynaptic DH neurons with BAPTA (10 mM) did not alter the effect of Naspm (100 μM) on the paired-pulse ratio. Our data provide evidence that presynaptic CP-AMPARs in nociceptive sensory synapses regulate the magnitude of PPD and that postsynaptic Ca(2+)-permeable glutamatergic receptors are not required for PPD induction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.neulet.2014.11.015 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulation of nociception by long-term potentiation of inhibitory postsynaptic currents from insular cortical parvalbumin-immunopositive neurons to pyramidal neurons.
Pain
January 2025
Department of Pharmacology, Nihon University School of Dentistry, Tokyo, Japan.
The insular cortex (IC) processes various sensory information, including nociception, from the trigeminal region. Repetitive nociceptive inputs from the orofacial area induce plastic changes in the IC. Parvalbumin-immunopositive neurons (PVNs) project to excitatory neurons (pyramidal neurons [PNs]), whose inputs strongly suppress the activities of PNs.
View Article and Find Full Text PDFNociceptor sodium channels shape subthreshold phase, upstroke, and shoulder of action potentials.
J Gen Physiol
March 2025
Institute for Neurophysiology, Uniklinik RWTH Aachen University, Aachen, Germany.
Voltage-gated sodium channels (VGSCs) in the peripheral nervous system shape action potentials (APs) and thereby support the detection of sensory stimuli. Most of the nine mammalian VGSC subtypes are expressed in nociceptors, but predominantly, three are linked to several human pain syndromes: while Nav1.7 is suggested to be a (sub-)threshold channel, Nav1.
View Article and Find Full Text PDFTreating episodic migraine with precision: the evolving landscape of targeted therapies driven by insights in disease biology.
Expert Opin Biol Ther
January 2025
University Medicine Greifswald, Greifswald, Germany.
Introduction: Migraine is a disabling neurological disorder with a complex neurobiology. It appears as a cyclic disorder of sensory processing, affecting multiple systems beyond nociception. Overlapping mechanisms, including dysfunctional processing of sensory input from brain structures are involved in the generation of attacks.
View Article and Find Full Text PDFAging negatively impacts central nervous system function; however, the cellular impact of aging in the peripheral nervous system remains poorly understood. Aged individuals are more likely to experience increased pain and slower recovery after trauma. Such injury can damage vulnerable peripheral axons of dorsal root ganglion (DRG) neurons resulting in somatosensory dysfunction.
View Article and Find Full Text PDFPhotobiomodulation therapy in diabetes: Benefits for pain relief, quality of life, and wound healing.
Photochem Photobiol
January 2025
Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Globally, 537 million people suffer from diabetes mellitus (DM), a condition often associated with sensory disturbances, wound development, and chronic pain, which significantly affects the quality of life and imposes a substantial economic burden. This study evaluated the effects of photobiomodulation (PBM) therapy on nociceptive and sensory changes in diabetic patients to understand pain manifestations and explore PBM's molecular mechanisms on wound healing. Twenty patients with type 2 DM underwent clinical assessments, completed pain and quality of life questionnaires, and had their pain sensitivity evaluated using the quantitative sensory test (QST).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!