The size- and fluorescence-based sorting of micro- and nanoscale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here, we demonstrate a disposable microfluidic particle sorter that enables high-throughput, on-demand counting and binary sorting of submicron particles and cells using either fluorescence or an electrically based determination of particle size. Size-based sorting uses a resistive pulse sensor integrated on-chip, whereas fluorescence-based discrimination is achieved using on-the-fly optical image capture and analysis. Following detection and analysis, the individual particles are deflected using a pair of piezoelectric actuators, directing the particles into one of two desired output channels; the main flow goes into a third waste channel. The integrated system can achieve sorting fidelities of better than 98%, and the mechanism can successfully count and actuate, on demand, more than 60,000 particles/min.
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http://dx.doi.org/10.1021/nl503783p | DOI Listing |
Electrophoresis
May 2024
State Key Laboratory of Precision Measuring Technology & Instruments, Tianjin University, Tianjin, P. R. China.
Particles, ranging from submicron to nanometer scale, can be broadly categorized into biological and non-biological types. Submicron-to-nanoscale bioparticles include various bacteria, viruses, liposomes, and exosomes. Non-biological particles cover various inorganic, metallic, and carbon-based particles.
View Article and Find Full Text PDFSci Adv
March 2024
Department of Chemistry, University of California-Riverside, Riverside, CA 92521, USA.
Extracellular vesicles (EVs) play important roles in cell-cell communication but are highly heterogeneous, and each vesicle has dimensions smaller than 200 nm with very limited amounts of cargos encapsulated. The technique of NanOstirBar (NOB)-EnabLed Single Particle Analysis (NOBEL-SPA) reported in the present work permits rapid inspection of single EV with high confidence by confocal fluorescence microscopy, thus enables colocalization assessment for selected protein and microRNA (miRNA) markers in the EVs produced by various cell lines, or present in clinical sera samples. EV subpopulations marked by the colocalization of unique protein and miRNA combinations were discovered to be able to detect early-stage (stage I or II) breast cancer (BC).
View Article and Find Full Text PDFNanoscale
February 2024
Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.
Manipulation, focusing, and separation of submicron- and nanoparticles such as extracellular vesicles (EVs), viruses and bacteria have broad applications in disease diagnostics and therapeutics. Viscoelastic microfluidic technology emerges as a promising technique, and it shows an unparalleled capacity to manipulate and separate submicron particles in a high resolution based on the elastic effects of non-Newtonian mediums. The maximum particle separation resolution for the reported state-of-the-art viscoelastic microfluidics is around 200 nm.
View Article and Find Full Text PDFChemosphere
December 2023
Faculty of Mining, Geology and Petroleum Engineering, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
This paper investigates occurrence of metal(loid)s, and size-dependent changes in their concentration in recent marine sediments from coastal and open-sea environments in the eastern Adriatic. Size fractionation of sediments was performed after removal of organic matter (OM), and the individual fractions, comprising particles below 8 μm, 4 μm, 2 μm, 1 μm and 0.45 μm, were analysed using HR ICP-MS.
View Article and Find Full Text PDFUltrasonics
January 2024
Department of Building Environment and Energy Engineering, The Hong Kong Polytechnic University, Hong Kong, China. Electronic address:
Acoustophoresis is an effective technique for particle manipulation. Acoustic radiation force scales with particle volume, enabling size separation. Yet, isolating sub-micron particles remains a challenge due to the acoustic streaming effect (ASE).
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