Aims: To evaluate the impact of human plasma-derived fibronectin (FN) on human subchondral mesenchymal progenitor cells regarding cell migration, proliferation, and chondrogenic differentiation.
Materials & Methods: Human subchondral mesenchymal progenitor cells were analyzed for their migration capacity upon treatment with human plasma-derived FN. Proliferation activity was evaluated by DNA content. For chondrogenesis, cells were cultured in high-density pellet cultures in the presence of FN, TGFβ3, and a combination thereof.
Results: Treatment of progenitors with FN significantly increased the number of migrating cells and elevated proliferative activity. Histological staining indicated formation of an extracellular matrix with type II collagen. Gene expression analysis gave no evidence for chondrogenic differentiation mediated by FN, but revealed a significant induction of type II collagen expression.
Conclusion: FN has a potential to recruit human subchondral mesenchymal progenitor cells, possibly supporting proliferation and matrix assembly in cartilage repair procedures using bioactive implants after microfracture treatment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2217/rme.14.40 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Introduction Total knee arthroplasty (TKA) is a widely accepted surgical intervention for patients with advanced knee osteoarthritis, aimed at reducing pain and improving functional mobility. Preoperative radiological evaluations, including assessments of joint space narrowing, osteophytes, varus/valgus deformities, and subchondral sclerosis, are essential for planning the surgery and predicting postoperative outcomes. Although extensive research has been conducted internationally, data focusing on populations in Saudi Arabia remain limited.
View Article and Find Full Text PDFSubchondral insufficiency fracture of the knee.
Orthopadie (Heidelb)
January 2025
Department of Orthopedic Surgery, Mayo Clinic, 200 First St. SW, 55905, Rochester, MN, USA.
Subchondral insufficiency fractures of the knee (SIFK) are a relatively common cause of knee pain, particularly in middle-aged and older adults. The SIFK is a type of stress fracture that occurs when excessive and repetitive or supraphysiologic loads are applied to subchondral bone [1]. Historically, this type of fracture was termed spontaneous osteonecrosis of the knee (SONK) until advances in MRI identified underlying fractures as well as meniscal deficiency as likely attributable etiologies.
View Article and Find Full Text PDFFixation Methods, Complications, and Outcomes After Primary Fixation of Isolated Chondral Fragments in the Knee: A Systematic Review.
Am J Sports Med
January 2025
Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri, USA.
Background: Knee injuries resulting in purely cartilaginous defects are rare, and controversy remains regarding the reliability of chondral-only fixation.
Purpose: To systematically review the literature for fixation methods and outcomes after primary fixation of chondral-only defects within the knee.
Study Design: Systematic review; Level of evidence, 5.
Osteochondral lesions of the talus involve injury to the articular cartilage and underlying subchondral bone. These lesions are difficult to treat because of the poor blood supply and poor regenerative capacity of the talar articular cartilage. It is important to provide a comprehensive overview of the clinical presentation, diagnostic tools, and nonsurgical and surgical treatment strategies for osteochondral lesions of the talus.
View Article and Find Full Text PDFArticle Synopsis
- The optimal treatment for B2 glenoid morphology in young, active osteoarthritis patients is debated, with various options like hemiarthroplasty, total shoulder arthroplasty (TSA), and reverse TSA available, each having pros and cons.
- Eccentric glenoid reaming can be effective but risks damaging the bone structure, potentially complicating future surgeries, while bone grafting, though beneficial for some, comes with high complication rates.
- Reverse TSA offers a more stable alternative when correcting version and inclination proves difficult, as it adapts better to glenoid deformities and maintains functionality with reduced bone stock.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!