AI Article Synopsis
- Cloned pigs created through somatic cell nuclear transfer (SCNT) experience higher rates of mid-gestation abortion compared to normal pigs.
- Research found that X-linked genes, especially Xist, show abnormal expression levels in aborted cloned pig fetuses.
- Analysis revealed hypomethylation of the Xist gene in both male and female aborted fetuses, suggesting that these expression issues may contribute to the increased abortion rates in cloned embryos.
Article Abstract
Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284668 | PMC |
| http://dx.doi.org/10.3390/ijms151221631 | DOI Listing |
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