AI Article Synopsis
- A study conducted in Nepal from 2009-2010 aimed to identify both known and unknown pathogens causing acute febrile illness, which is commonly treated without a proper diagnosis in resource-poor settings.
- Diagnostic tests and advanced sequencing techniques were used on samples from patients, focusing on pathogens like West Nile virus (WNV).
- Out of 2,046 patients tested, only 14 were positive for WNV, with two confirmed cases, indicating that WNV lineage 1 is present in Nepal and should be considered in future illness diagnoses.
Article Abstract
Background: Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepal.
Method: Study methods included diagnostic testing and preliminary ELISA screening of acute and convalescent samples for diseases both known and unknown to be circulating in Nepal, including West Nile virus (WNV). The molecular assays including Polymerase Chain Reaction (PCR), Sanger sequencing and ultra deep sequencing on MiSeq Illumina Platform were conducted to further confirm the presence of WNV.
Results: The study enrolled 2,046 patients presenting undifferentiated febrile illness with unknown etiology. Sera from 14 out of 2,046 patients were tested positive for west nile virus (WNV) by nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Only two out of 14 cases were confirmed for the presence of WNV by sequencing and identified as WNV lineage 1 phylogentically. The two patients were adult males with fever and no neurological symptoms from Kathmandu and Bharatpur, Nepal.
Conclusion: Two out of 2,046 serum samples contained fragments of WNV genome resembling WNV lineage 1, which is evidence of the continued spread of WNV which should be considered a possible illness cause in Nepal.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265323 | PMC |
| http://dx.doi.org/10.1186/s12879-014-0606-0 | DOI Listing |
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